The single-cell epigenomic and transcriptional landscape of immunity to influenza vaccination.
Cell
; 184(15): 3915-3935.e21, 2021 07 22.
Article
in English
| MEDLINE | ID: covidwho-1283262
ABSTRACT
Emerging evidence indicates a fundamental role for the epigenome in immunity. Here, we mapped the epigenomic and transcriptional landscape of immunity to influenza vaccination in humans at the single-cell level. Vaccination against seasonal influenza induced persistently diminished H3K27ac in monocytes and myeloid dendritic cells (mDCs), which was associated with impaired cytokine responses to Toll-like receptor stimulation. Single-cell ATAC-seq analysis revealed an epigenomically distinct subcluster of monocytes with reduced chromatin accessibility at AP-1-targeted loci after vaccination. Similar effects were observed in response to vaccination with the AS03-adjuvanted H5N1 pandemic influenza vaccine. However, this vaccine also stimulated persistently increased chromatin accessibility at interferon response factor (IRF) loci in monocytes and mDCs. This was associated with elevated expression of antiviral genes and heightened resistance to the unrelated Zika and Dengue viruses. These results demonstrate that vaccination stimulates persistent epigenomic remodeling of the innate immune system and reveal AS03's potential as an epigenetic adjuvant.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
Influenza Vaccines
/
Vaccination
/
Single-Cell Analysis
/
Epigenomics
/
Immunity
Type of study:
Randomized controlled trials
Topics:
Vaccines
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Young adult
Language:
English
Journal:
Cell
Year:
2021
Document Type:
Article
Affiliation country:
J.cell.2021.05.039
Similar
MEDLINE
...
LILACS
LIS