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A novel G-quadruplex aptamer-based spike trimeric antigen test for the detection of SARS-CoV-2.
Gupta, Ankit; Anand, Anjali; Jain, Neha; Goswami, Sandeep; Anantharaj, Anbalagan; Patil, Sharanabasava; Singh, Rahul; Kumar, Amit; Shrivastava, Tripti; Bhatnagar, Shinjini; Medigeshi, Guruprasad R; Sharma, Tarun Kumar.
  • Gupta A; Aptamer Technology and Diagnostics Laboratory, Multidisciplinary Clinical and Translational Research, Translational Health Science and Technology Institute, Faridabad, Haryana 121001, India.
  • Anand A; Aptamer Technology and Diagnostics Laboratory, Multidisciplinary Clinical and Translational Research, Translational Health Science and Technology Institute, Faridabad, Haryana 121001, India.
  • Jain N; Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Simrol, Madhya Pradesh 453552, India.
  • Goswami S; Infection and Immunology, Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad, Haryana 121001, India.
  • Anantharaj A; Translational Health Science and Technology Institute, Faridabad, Haryana 121001, India.
  • Patil S; Translational Health Science and Technology Institute, Faridabad, Haryana 121001, India.
  • Singh R; Raja Ramanna Centre for Advanced Technology, Indore, Madhya Pradesh 452013, India.
  • Kumar A; Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Simrol, Madhya Pradesh 453552, India.
  • Shrivastava T; Infection and Immunology, Translational Health Science & Technology Institute, NCR Biotech Science Cluster, Faridabad, Haryana 121001, India.
  • Bhatnagar S; Translational Health Science and Technology Institute, Faridabad, Haryana 121001, India.
  • Medigeshi GR; Translational Health Science and Technology Institute, Faridabad, Haryana 121001, India.
  • Sharma TK; Aptamer Technology and Diagnostics Laboratory, Multidisciplinary Clinical and Translational Research, Translational Health Science and Technology Institute, Faridabad, Haryana 121001, India.
Mol Ther Nucleic Acids ; 26: 321-332, 2021 Dec 03.
Article in English | MEDLINE | ID: covidwho-1284428
ABSTRACT
The recent SARS-CoV-2 outbreak has been declared a global health emergency. It will take years to vaccinate the whole population to protect them from this deadly virus, hence the management of SARS-CoV-2 largely depends on the widespread availability of an accurate diagnostic test. Toward addressing the unmet need of a reliable diagnostic test in the current work by utilizing the power of Systematic Evolution of Ligands by EXponential enrichment, a 44-mer G-quadruplex-forming DNA aptamer against spike trimer antigen of SARS-CoV-2 was identified. The lead aptamer candidate (S14) was characterized thoroughly for its binding, selectivity, affinity, structure, and batch-to-batch variability by utilizing various biochemical, biophysical, and in silico techniques. S14 has demonstrated a low nanomolar KD, confirming its tight binding to a spike antigen of SARS-CoV-2. S14 can detect as low as 2 nM of antigen. The clinical evaluation of S14 aptamer on nasopharyngeal swab specimens (n = 232) has displayed a highly discriminatory response between SARS-CoV-2 infected individuals from the non-infected one with a sensitivity and specificity of ∼91% and 98%, respectively. Importantly, S14 aptamer-based test has evinced a comparable performance with that of RT-PCR-based assay. Altogether, this study established the utility of aptamer technology for the detection of SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies / Prognostic study / Systematic review/Meta Analysis Topics: Vaccines Language: English Journal: Mol Ther Nucleic Acids Year: 2021 Document Type: Article Affiliation country: J.omtn.2021.06.014

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study / Experimental Studies / Prognostic study / Systematic review/Meta Analysis Topics: Vaccines Language: English Journal: Mol Ther Nucleic Acids Year: 2021 Document Type: Article Affiliation country: J.omtn.2021.06.014