Selenium atom on phosphate enhances specificity and sensitivity of DNA polymerization and detection.
J Mater Chem B
; 9(28): 5636-5644, 2021 07 21.
Article
in English
| MEDLINE | ID: covidwho-1291040
ABSTRACT
DNA polymerization is of high specificity in vivo. However, its specificity is much lower in vitro, which limits advanced applications of DNA polymerization in ultrasensitive nucleic acid detection. Herein, we report a unique mechanism of single selenium-atom modified dNTP (dNTPαSe) to enhance polymerization specificity. We have found that both dNTPαSe (approximately 660 fold) and Se-DNA (approximately 2.8 fold) have lower binding affinity to DNA polymerase than canonical ones, and the Se-DNA duplex has much lower melting-temperature (Tm) than the corresponding canonical DNA duplex. The reduced affinity and Tm can destabilize the substrate-primer-template-enzyme assembly, thereby largely slowing down the mismatch of DNA polymerization and enhancing the amplification specificity and in turn detection sensitivity. Furthermore, the Se-strategy enables us to develop the selenium enhanced specific isothermal amplification (SEA) for nucleic acid detection with high specificity and sensitivity (up to detection of single-digit copies), allowing convenient detection of clinical HPV and COVID-19 viruses in the low-copy number. Clearly, we have discovered the exciting mechanism for enhancing DNA polymerization accuracy, amplification specificity and detection sensitivity by SEA, up to two orders of magnitude higher.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Phosphates
/
Selenium
/
DNA, Viral
/
Human papillomavirus 16
/
SARS-CoV-2
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
J Mater Chem B
Year:
2021
Document Type:
Article
Affiliation country:
D1tb00428j
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