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Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay.
Algaissi, Abdullah; Hashem, Anwar M.
  • Algaissi A; Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA.
  • Hashem AM; Department of Medical Laboratories Technology, College of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia.
Methods Mol Biol ; 2099: 107-116, 2020.
Article in English | MEDLINE | ID: covidwho-1292549
ABSTRACT
The microneutralization (MN) assay is a standard and important technique in virology, immunology, and epidemiology. It is a highly specific and sensitive assay for evaluating virus-specific neutralizing antibodies (nAbs) in human and animal sera. It provides the most precise answer to whether or not an individual or animal has antibodies that can neutralize or inhibit the infectivity of a specific virus strain. However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). In this chapter, we describe the isolation, amplification, and titration of MERS-CoV, as well as detailed MN assay to measure nAb levels in sera from different mammalian species.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus Infections / Antibodies, Neutralizing / Middle East Respiratory Syndrome Coronavirus Type of study: Experimental Studies / Prognostic study Limits: Animals / Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2020 Document Type: Article Affiliation country: 978-1-0716-0211-9_9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus Infections / Antibodies, Neutralizing / Middle East Respiratory Syndrome Coronavirus Type of study: Experimental Studies / Prognostic study Limits: Animals / Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2020 Document Type: Article Affiliation country: 978-1-0716-0211-9_9