SARS-CoV-2 Spike protein enhances ACE2 expression via facilitating Interferon effects in bronchial epithelium.
Immunol Lett
; 237: 33-41, 2021 09.
Article
in English
| MEDLINE | ID: covidwho-1293862
ABSTRACT
OBJECTIVE:
In this study, we focused on the interaction between SARS-CoV-2 and host Type I Interferon (IFN) response, so as to identify whether IFN effects could be influenced by the products of SARS-CoV-2.METHODS:
All the structural and non-structural proteins of SARS-CoV-2 were transfected and overexpressed in the bronchial epithelial cell line BEAS-2B respectively, and typical antiviral IFN-stimulated gene (ISG) ISG15 expression was detected by qRT-PCR. RNA-seq based transcriptome analysis was performed between control and Spike (S) protein-overexpressed BEAS-2B cells. The expression of ACE2 and IFN effector JAK-STAT signaling activation were detected in control and S protein-overexpressed BEAS-2B cells by qRT-PCR or/and Western blot respectively. The interaction between S protein with STAT1 and STAT2, and the association between JAK1 with downstream STAT1 and STAT2 were measured in BEAS-2B cells by co-immunoprecipitation (co-IP).RESULTS:
S protein could activate IFN effects and downstream ISGs expression. By transcriptome analysis, overexpression of S protein induced a set of genes expression, including series of ISGs and the SARS-CoV-2 receptor ACE2. Mechanistically, S protein enhanced the association between the upstream JAK1 and downstream STAT1 and STAT2, so as to promote STAT1 and STAT2 phosphorylation and ACE2 expression.CONCLUSION:
SARS-CoV-2 S protein enhances ACE2 expression via facilitating IFN effects, which may help its infection.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Bronchi
/
Epithelial Cells
/
Spike Glycoprotein, Coronavirus
/
Interferon alpha-2
/
Angiotensin-Converting Enzyme 2
/
SARS-CoV-2
/
COVID-19
Type of study:
Experimental Studies
Limits:
Humans
Language:
English
Journal:
Immunol Lett
Year:
2021
Document Type:
Article
Affiliation country:
J.imlet.2021.06.008
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