A comprehensive library of fluorescent constructs of SARS-CoV-2 proteins and their initial characterisation in different cell types.
Biol Cell
; 113(7): 311-328, 2021 Jul.
Article
in English
| MEDLINE | ID: covidwho-1294968
ABSTRACT
BACKGROUND INFORMATION Comprehensive libraries of plasmids for SARS-CoV-2 proteins with various tags (e.g., Strep, HA, Turbo) are now available. They enable the identification of numerous potential protein-protein interactions between the SARS-CoV-2 virus and host proteins. RESULTS:
We present here a large library of SARS CoV-2 protein constructs fused with green and red fluorescent proteins and their initial characterisation in various human cell lines including lung epithelial cell models (A549, BEAS-2B), as well as in budding yeast. The localisation of a few SARS-CoV-2 proteins matches their proposed interactions with host proteins. These include the localisation of Nsp13 to the centrosome, Orf3a to late endosomes and Orf9b to mitochondria. CONCLUSIONS ANDSIGNIFICANCE:
This library should facilitate further cellular investigations, notably by imaging techniques.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viral Proteins
/
Peptide Library
/
SARS-CoV-2
/
COVID-19
Type of study:
Randomized controlled trials
Limits:
Humans
Language:
English
Journal:
Biol Cell
Year:
2021
Document Type:
Article
Affiliation country:
Boc.202000158
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