Your browser doesn't support javascript.
Development of a high-sensitivity ELISA detecting IgG, IgA and IgM antibodies to the SARS-CoV-2 spike glycoprotein in serum and saliva.
Faustini, Sian E; Jossi, Sian E; Perez-Toledo, Marisol; Shields, Adrian M; Allen, Joel D; Watanabe, Yasunori; Newby, Maddy L; Cook, Alex; Willcox, Carrie R; Salim, Mahboob; Goodall, Margaret; Heaney, Jennifer L; Marcial-Juarez, Edith; Morley, Gabriella L; Torlinska, Barbara; Wraith, David C; Veenith, Tonny V; Harding, Stephen; Jolles, Stephen; Ponsford, Mark J; Plant, Tim; Huissoon, Aarnoud; O'Shea, Matthew K; Willcox, Benjamin E; Drayson, Mark T; Crispin, Max; Cunningham, Adam F; Richter, Alex G.
  • Faustini SE; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Jossi SE; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Perez-Toledo M; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Shields AM; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Allen JD; School of Biological Sciences, University of Southampton, Southampton, UK.
  • Watanabe Y; School of Biological Sciences, University of Southampton, Southampton, UK.
  • Newby ML; Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford, UK.
  • Cook A; School of Biological Sciences, University of Southampton, Southampton, UK.
  • Willcox CR; Binding Site Group Ltd, Birmingham, UK.
  • Salim M; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Goodall M; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Heaney JL; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Marcial-Juarez E; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Morley GL; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Torlinska B; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
  • Wraith DC; Institute of Applied Health Research, University of Birmingham, Birmingham, UK.
  • Veenith TV; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Harding S; Department of Critical Care Medicine, University Hospitals Birmingham NHS Trust, Birmingham, UK.
  • Jolles S; Binding Site Group Ltd, Birmingham, UK.
  • Ponsford MJ; Immunodeficiency Centre for Wales, Cardiff, UK.
  • Plant T; Immunodeficiency Centre for Wales, Cardiff, UK.
  • Huissoon A; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • O'Shea MK; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Willcox BE; Department of Immunology, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.
  • Drayson MT; Institute of Microbiology and Infection, University of Birmingham, Birmingham, UK.
  • Crispin M; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Cunningham AF; Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.
  • Richter AG; School of Biological Sciences, University of Southampton, Southampton, UK.
Immunology ; 164(1): 135-147, 2021 09.
Article in English | MEDLINE | ID: covidwho-1295026
Semantic information from SemMedBD (by NLM)
1. saliva LOCATION_OF spike glycoprotei
Subject
saliva
Predicate
LOCATION_OF
Object
spike glycoprotei
2. Serum LOCATION_OF spike glycoprotei
Subject
Serum
Predicate
LOCATION_OF
Object
spike glycoprotei
3. Antibodies ASSOCIATED_WITH Disease
Subject
Antibodies
Predicate
ASSOCIATED_WITH
Object
Disease
4. Antibodies TREATS Severe disease
Subject
Antibodies
Predicate
TREATS
Object
Severe disease
5. Antibodies TREATS hospitalized patients
Subject
Antibodies
Predicate
TREATS
Object
hospitalized patients
6. Severe disease PROCESS_OF hospitalized patients
Subject
Severe disease
Predicate
PROCESS_OF
Object
hospitalized patients
7. Assay USES Antibodies
Subject
Assay
Predicate
USES
Object
Antibodies
8. Detection TREATS Persons
Subject
Detection
Predicate
TREATS
Object
Persons
9. Serum LOCATION_OF Anti-Antibodies
Subject
Serum
Predicate
LOCATION_OF
Object
Anti-Antibodies
10. High Level PROCESS_OF Persons
Subject
High Level
Predicate
PROCESS_OF
Object
Persons
11. saliva LOCATION_OF Immunoglobulin M|CD40LG
Subject
saliva
Predicate
LOCATION_OF
Object
Immunoglobulin M|CD40LG
12. saliva LOCATION_OF anti-IgG
Subject
saliva
Predicate
LOCATION_OF
Object
anti-IgG
13. saliva LOCATION_OF immunoglobulin
Subject
saliva
Predicate
LOCATION_OF
Object
immunoglobulin
14. Antibody Formation AFFECTS Virus
Subject
Antibody Formation
Predicate
AFFECTS
Object
Virus
15. saliva LOCATION_OF spike glycoprotein, SARS-CoV
Subject
saliva
Predicate
LOCATION_OF
Object
spike glycoprotein, SARS-CoV
16. Serum LOCATION_OF spike glycoprotein, SARS-CoV
Subject
Serum
Predicate
LOCATION_OF
Object
spike glycoprotein, SARS-CoV
17. Antibodies ASSOCIATED_WITH Disease
Subject
Antibodies
Predicate
ASSOCIATED_WITH
Object
Disease
18. Antibodies TREATS Severe disease
Subject
Antibodies
Predicate
TREATS
Object
Severe disease
19. Antibodies TREATS hospitalized patients
Subject
Antibodies
Predicate
TREATS
Object
hospitalized patients
20. Severe disease PROCESS_OF hospitalized patients
Subject
Severe disease
Predicate
PROCESS_OF
Object
hospitalized patients
21. Assay USES Antibodies
Subject
Assay
Predicate
USES
Object
Antibodies
22. Detection TREATS Persons
Subject
Detection
Predicate
TREATS
Object
Persons
23. Serum LOCATION_OF Anti-Antibodies
Subject
Serum
Predicate
LOCATION_OF
Object
Anti-Antibodies
24. High Level PROCESS_OF Persons
Subject
High Level
Predicate
PROCESS_OF
Object
Persons
25. saliva LOCATION_OF Immunoglobulin M|CD40LG
Subject
saliva
Predicate
LOCATION_OF
Object
Immunoglobulin M|CD40LG
26. saliva LOCATION_OF anti-IgG
Subject
saliva
Predicate
LOCATION_OF
Object
anti-IgG
27. saliva LOCATION_OF immunoglobulin A, human
Subject
saliva
Predicate
LOCATION_OF
Object
immunoglobulin A, human
28. Antibody Formation AFFECTS Virus
Subject
Antibody Formation
Predicate
AFFECTS
Object
Virus
ABSTRACT
Detecting antibody responses during and after SARS-CoV-2 infection is essential in determining the seroepidemiology of the virus and the potential role of antibody in disease. Scalable, sensitive and specific serological assays are essential to this process. The detection of antibody in hospitalized patients with severe disease has proven relatively straightforward; detecting responses in subjects with mild disease and asymptomatic infections has proven less reliable. We hypothesized that the suboptimal sensitivity of antibody assays and the compartmentalization of the antibody response may contribute to this effect. We systematically developed an ELISA, optimizing different antigens and amplification steps, in serum and saliva from non-hospitalized SARS-CoV-2-infected subjects. Using trimeric spike glycoprotein, rather than nucleocapsid, enabled detection of responses in individuals with low antibody responses. IgG1 and IgG3 predominate to both antigens, but more anti-spike IgG1 than IgG3 was detectable. All antigens were effective for detecting responses in hospitalized patients. Anti-spike IgG, IgA and IgM antibody responses were readily detectable in saliva from a minority of RT-PCR confirmed, non-hospitalized symptomatic individuals, and these were mostly subjects who had the highest levels of anti-spike serum antibodies. Therefore, detecting antibody responses in both saliva and serum can contribute to determining virus exposure and understanding immune responses after SARS-CoV-2 infection.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Immunoglobulin A / Immunoglobulin G / Immunoglobulin M / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral Subject: Immunoglobulin A / Immunoglobulin G / Immunoglobulin M / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Language: English Journal: Immunology Clinical aspect: Diagnosis Year: 2021

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Immunoglobulin A / Immunoglobulin G / Immunoglobulin M / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral Subject: Immunoglobulin A / Immunoglobulin G / Immunoglobulin M / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study Language: English Journal: Immunology Clinical aspect: Diagnosis Year: 2021
...