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Seropositive Reaction Rates of 9 B-Cell Epitopes of the SARS-CoV-2 Spike Protein and the Relationship between the Epitopes and Neutralizing Antibody.
Zhu, Li; Zhang, Yunwen; Yang, Zhengrong; Li, Baisheng; Feng, Tiejian; Zou, Xuan; He, Jianfan; He, Taiping; Li, Junling; Liu, Ning; Li, Wei; Wang, Xiaohui.
  • Zhu L; School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
  • Zhang Y; Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
  • Yang Z; West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.
  • Li B; Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
  • Feng T; Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
  • Zou X; Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
  • He J; Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
  • He T; Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
  • Li J; School of Public Health, Guangdong Medical University, Zhanjiang, China.
  • Liu N; School of Public Health, Guangdong Medical University, Zhanjiang, China.
  • Li W; Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
  • Wang X; School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
Intervirology ; 65(1): 29-36, 2022.
Article in English | MEDLINE | ID: covidwho-1299259
ABSTRACT

OBJECTIVE:

The aim of the study was to analyze the relationship between serum antibody and neutralizing antibody titers in convalescent coronavirus disease 2019 (COVID-19) patients with different disease severities, and the seropositive reaction rates of 9 reported B-cell epitopes of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

METHODS:

Serum IgG and total antibody titers of 165 convalescent COVID-19 patients were determined by chemiluminescence, the serum neutralization antibody titers were determined by microneutralization assay, and the S/CO values of 9 peptides were detected by indirect enzyme-linked immunosorbent assay. Correlations between the aforementioned indexes were statistically analyzed, and differences in patients with different diseases severities were evaluated.

RESULTS:

IgG, total antibody, and neutralizing antibody titers increased with disease severity. The positive rate of the receptor-binding region (RBD) was 100%, and the average positive rate for all the 9 peptides was above 50% in 165 patients. IDf showed the highest rate of positivity (86.06%), with a rate of 95% for the (IDf + IDa) pattern. Moreover, S/CO values of RBD and mix (IDh) were significantly correlated with IgG, total antibody titers, and neutralizing antibody titers (p < 0.001), whereas the S/CO values for other 8 peptides showed no obvious correlation.

CONCLUSION:

In this study, a large sample was used to confirm that the peptide IDf had a high positive reaction rate for all patients (86.06%) and also had the highest detection rate in asymptomatic patients (86.67%). Only long peptide and mixed peptide showed correlation with neutralizing antibody titers, suggesting that the ability of SARS-CoV-2 antibody to neutralize virus infectivity may require the interaction of multiple sites.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Epitopes, B-Lymphocyte / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Antibodies, Viral Type of study: Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Intervirology Year: 2022 Document Type: Article Affiliation country: 000517717

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Epitopes, B-Lymphocyte / Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Antibodies, Viral Type of study: Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Intervirology Year: 2022 Document Type: Article Affiliation country: 000517717