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A mask-based diagnostic platform for point-of-care screening of Covid-19.
Daniels, John; Wadekar, Shekhar; DeCubellis, Ken; Jackson, George W; Chiu, Alexander S; Pagneux, Quentin; Saada, Hiba; Engelmann, Ilka; Ogiez, Judith; Loze-Warot, Delphine; Boukherroub, Rabah; Szunerits, Sabine.
  • Daniels J; Diagmetrics Inc., 30 Renees Way Madison, Connecticut, 06443, USA.
  • Wadekar S; Diagmetrics Inc., 30 Renees Way Madison, Connecticut, 06443, USA.
  • DeCubellis K; Diagmetrics Inc., 30 Renees Way Madison, Connecticut, 06443, USA.
  • Jackson GW; Base Pair Biotechnologies, Inc., 8619 Broadway St., Suite 100, Pearland, TX, USA.
  • Chiu AS; Base Pair Biotechnologies, Inc., 8619 Broadway St., Suite 100, Pearland, TX, USA.
  • Pagneux Q; Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000, Lille, France.
  • Saada H; Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000, Lille, France.
  • Engelmann I; Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000, Lille, France.
  • Ogiez J; Univ Lille, CHU Lille, Laboratoire de Virologie ULR3610, F-59000, Lille, France.
  • Loze-Warot D; CerbaHealthCare Biomedical Laboratory, CERBALLIANCE Lille, 17/24 Rue de La Digue, 59000, Lille, France.
  • Boukherroub R; Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000, Lille, France.
  • Szunerits S; Univ. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520 - IEMN, F-59000, Lille, France. Electronic address: sabine.szunerits@univ-lille.fr.
Biosens Bioelectron ; 192: 113486, 2021 Nov 15.
Article in English | MEDLINE | ID: covidwho-1300634
ABSTRACT
Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200-500 µL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL-1 in cultured SARS-CoV-2 suspensions. Using a "turn off" assay via ferrocenemethanol redox mediator, results about the infectivity state of the patient are obtained in 10 min.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2021 Document Type: Article Affiliation country: J.bios.2021.113486

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / COVID-19 Type of study: Diagnostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2021 Document Type: Article Affiliation country: J.bios.2021.113486