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Cross-validation of ELISA and a portable surface plasmon resonance instrument for IgG antibody serology with SARS-CoV-2 positive individuals.
Djaileb, Abdelhadi; Hojjat Jodaylami, Maryam; Coutu, Julien; Ricard, Pierre; Lamarre, Mathieu; Rochet, Léa; Cellier-Goetghebeur, Stella; Macaulay, Devin; Charron, Benjamin; Lavallée, Étienne; Thibault, Vincent; Stevenson, Keisean; Forest, Simon; Live, Ludovic S; Abonnenc, Nanouk; Guedon, Anthony; Quessy, Patrik; Lemay, Jean-François; Farnós, Omar; Kamen, Amine; Stuible, Matthew; Gervais, Christian; Durocher, Yves; Cholette, François; Mesa, Christine; Kim, John; Cayer, Marie-Pierre; de Grandmont, Marie-Joëlle; Brouard, Danny; Trottier, Sylvie; Boudreau, Denis; Pelletier, Joelle N; Masson, Jean-Francois.
  • Djaileb A; Department of Chemistry, Department of Biochemistry and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada. joelle.pelletier@umontreal.ca and Affinité Instruments, 1250 rue Gu
  • Hojjat Jodaylami M; Department of Chemistry, Quebec Centre for Advanced Materials (QCAM), Regroupement Québécois sur les Matériaux de Pointe (RQMP), and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA), Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada.
  • Coutu J; Department of Chemistry, Quebec Centre for Advanced Materials (QCAM), Regroupement Québécois sur les Matériaux de Pointe (RQMP), and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA), Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada.
  • Ricard P; Department of Chemistry, Quebec Centre for Advanced Materials (QCAM), Regroupement Québécois sur les Matériaux de Pointe (RQMP), and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA), Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada.
  • Lamarre M; Department of Chemistry and Centre for Optics, Photonics and Lasers (COPL), Université Laval, 1045, av. de la Médecine, Québec City, Québec G1V 0A6, Canada.
  • Rochet L; Department of Chemistry, Department of Biochemistry and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada. joelle.pelletier@umontreal.ca.
  • Cellier-Goetghebeur S; Department of Chemistry, Department of Biochemistry and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada. joelle.pelletier@umontreal.ca.
  • Macaulay D; Department of Chemistry and Centre for Optics, Photonics and Lasers (COPL), Université Laval, 1045, av. de la Médecine, Québec City, Québec G1V 0A6, Canada.
  • Charron B; Department of Chemistry, Quebec Centre for Advanced Materials (QCAM), Regroupement Québécois sur les Matériaux de Pointe (RQMP), and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA), Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada.
  • Lavallée É; Department of Chemistry, Department of Biochemistry and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada. joelle.pelletier@umontreal.ca.
  • Thibault V; Department of Chemistry, Quebec Centre for Advanced Materials (QCAM), Regroupement Québécois sur les Matériaux de Pointe (RQMP), and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA), Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada.
  • Stevenson K; Department of Chemistry, Quebec Centre for Advanced Materials (QCAM), Regroupement Québécois sur les Matériaux de Pointe (RQMP), and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA), Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada.
  • Forest S; Department of Chemistry, Quebec Centre for Advanced Materials (QCAM), Regroupement Québécois sur les Matériaux de Pointe (RQMP), and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA), Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada.
  • Live LS; Affinité Instruments, 1250 rue Guy, Suite 600, Montréal, Québec H3H 2L3, Canada.
  • Abonnenc N; CNETE and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Cégep de Shawinigan, 2263 Avenue du Collège, Shawinigan, Québec G9N 6 V8, Canada.
  • Guedon A; CNETE and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Cégep de Shawinigan, 2263 Avenue du Collège, Shawinigan, Québec G9N 6 V8, Canada.
  • Quessy P; CNETE and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Cégep de Shawinigan, 2263 Avenue du Collège, Shawinigan, Québec G9N 6 V8, Canada.
  • Lemay JF; CNETE and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Cégep de Shawinigan, 2263 Avenue du Collège, Shawinigan, Québec G9N 6 V8, Canada.
  • Farnós O; Department of Bioengineering, McGill University McConnell Engineering Building, 3480 University Street, Montreal, Québec H3A 0E9, Canada.
  • Kamen A; Department of Bioengineering, McGill University McConnell Engineering Building, 3480 University Street, Montreal, Québec H3A 0E9, Canada.
  • Stuible M; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.
  • Gervais C; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.
  • Durocher Y; Mammalian Cell Expression, Human Health Therapeutics Research Centre, National Research Council Canada, Montréal, Québec, Canada.
  • Cholette F; National Laboratory for HIV Reference Services, National Microbiology Laboratory at the JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Canada and Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Canada.
  • Mesa C; National Laboratory for HIV Reference Services, National Microbiology Laboratory at the JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Canada.
  • Kim J; National Laboratory for HIV Reference Services, National Microbiology Laboratory at the JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, Canada.
  • Cayer MP; Héma-Québec, Affaires médicales et innovation, 1070, avenue des Sciences-de-la-Vie, Québec City, G1V 5C3, Québec, Canada.
  • de Grandmont MJ; Héma-Québec, Affaires médicales et innovation, 1070, avenue des Sciences-de-la-Vie, Québec City, G1V 5C3, Québec, Canada.
  • Brouard D; Héma-Québec, Affaires médicales et innovation, 1070, avenue des Sciences-de-la-Vie, Québec City, G1V 5C3, Québec, Canada.
  • Trottier S; Centre de recherche du Centre hospitalier universitaire de Québec and Département de microbiologie-infectiologie et d'immunologie, Université Laval 2705, boulevard Laurier, Québec City, Québec, Canada G1V 4G2.
  • Boudreau D; Department of Chemistry and Centre for Optics, Photonics and Lasers (COPL), Université Laval, 1045, av. de la Médecine, Québec City, Québec G1V 0A6, Canada.
  • Pelletier JN; Department of Chemistry, Department of Biochemistry and PROTEO, The Québec Network for Research on Protein Function, Engineering and Applications, Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada. joelle.pelletier@umontreal.ca.
  • Masson JF; Department of Chemistry, Quebec Centre for Advanced Materials (QCAM), Regroupement Québécois sur les Matériaux de Pointe (RQMP), and Centre interdisciplinaire de recherche sur le cerveau et l'apprentissage (CIRCA), Université de Montréal, CP 6128 Succ. Centre-Ville, Montreal, Québec H3C 3J7, Canada.
Analyst ; 146(15): 4905-4917, 2021 Jul 26.
Article in English | MEDLINE | ID: covidwho-1305374
ABSTRACT
We report on the development of surface plasmon resonance (SPR) sensors and matching ELISAs for the detection of nucleocapsid and spike antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in human serum, plasma and dried blood spots (DBS). When exposed to SARS-CoV-2 or a vaccine against SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the fraction of the population potentially immunized against SARS-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with various sources of SARS-CoV-2 recombinant proteins expressed in different cell lines detected human anti-SARS-CoV-2 IgG antibodies in clinical samples. Nucleocapsid expressed in different cell lines did not significantly change the sensitivity of the assays, whereas the use of a CHO cell line to express spike ectodomain led to excellent performance. This bioassay was performed on a portable SPR instrument capable of measuring 4 biological samples within 30 minutes of sample/sensor contact and the chip could be regenerated at least 9 times. Multi-site validation was then performed with in-house and commercial ELISA, which revealed excellent cross-correlations with Pearson's coefficients exceeding 0.85 in all cases, for measurements in DBS and plasma. This strategy paves the way to point-of-care and rapid testing for antibodies in the context of viral infection and vaccine efficacy monitoring.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Humans Language: English Journal: Analyst Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study / Randomized controlled trials Topics: Vaccines Limits: Humans Language: English Journal: Analyst Year: 2021 Document Type: Article