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RNA-dependent assembly of chimeric antigen nanoparticles as an efficient H5N1 pre-pandemic vaccine platform.
Lim, Jongkwan; Cheong, Yucheol; Kim, Young-Seok; Chae, Wonil; Hwang, Beom Jeung; Lee, Jinhee; Jang, Yo Han; Roh, Young Hoon; Seo, Sang-Uk; Seong, Baik L.
  • Lim J; Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.
  • Cheong Y; Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.
  • Kim YS; Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.
  • Chae W; Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.
  • Hwang BJ; Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.
  • Lee J; Department of Integrated OMICS for Biomedical Science, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea.
  • Jang YH; Department of Biological Sciences and Biotechnology, College of Life Sciences and Biotechnology, Andong National University, Andong, Republic of Korea.
  • Roh YH; Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea. Electronic address: yr36@yonsei.ac.kr.
  • Seo SU; Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: suseo@catholic.ac.kr.
  • Seong BL; Department of Biotechnology, College of Life Sciences and Biotechnology, Yonsei University, Seoul, Republic of Korea; Department of Microbiology, College of Medicine, Yonsei University, Seoul, Republic of Korea; Vaccine Innovative Technology Alliance-Korea, Yonsei University, Seoul, Republic of Kore
Nanomedicine ; 37: 102438, 2021 10.
Article in English | MEDLINE | ID: covidwho-1306447
ABSTRACT
Highly pathogenic avian influenza viruses (HPAIVs) pose a significant threat to human health, with high mortality rates, and require effective vaccines. We showed that, harnessed with novel RNA-mediated chaperone function, hemagglutinin (HA) of H5N1 HPAIV could be displayed as an immunologically relevant conformation on self-assembled chimeric nanoparticles (cNP). A tri-partite monomeric antigen was designed including i) an RNA-interaction domain (RID) as a docking tag for RNA to enable chaperna function (chaperna chaperone + RNA), ii) globular head domain (gd) of HA as a target antigen, and iii) ferritin as a scaffold for 24 mer-assembly. The immunization of mice with the nanoparticles (~46 nm) induced a 25-30 fold higher neutralizing capacity of the antibody and provided cross-protection from homologous and heterologous lethal challenges. This study suggests that cNP assembly is conducive to eliciting antibodies against the conserved region in HA, providing potent and broad protective efficacy.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA / Influenza Vaccines / Hemagglutinin Glycoproteins, Influenza Virus / Influenza A Virus, H5N1 Subtype / Influenza in Birds Type of study: Randomized controlled trials Topics: Vaccines Limits: Animals / Humans Language: English Journal: Nanomedicine Journal subject: Biotechnology Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA / Influenza Vaccines / Hemagglutinin Glycoproteins, Influenza Virus / Influenza A Virus, H5N1 Subtype / Influenza in Birds Type of study: Randomized controlled trials Topics: Vaccines Limits: Animals / Humans Language: English Journal: Nanomedicine Journal subject: Biotechnology Year: 2021 Document Type: Article