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SARS-CoV-2 spike linear epitope scanning via a peptide microarray through sera profiling.
Li, Yang; Lai, Dan-Yun; Tao, Sheng-Ce.
  • Li Y; Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
  • Lai DY; College of Life Science, Nankai University, Tianjin 300071, China.
  • Tao SC; Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
STAR Protoc ; 2(3): 100707, 2021 09 17.
Article in English | MEDLINE | ID: covidwho-1307268
ABSTRACT
Host humoral immunological response plays an essential role in protection against pathogens. Identification of B-cell epitopes on antigens is required for accurate diagnosis and vaccine development. To map SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) spike linear epitopes, we developed a protocol of profiling sera from patients with COVID-19 (coronavirus disease 2019) via a peptide microarray designed according to spike protein. The protocol is also applicable for other antigens or sample types. This protocol is rapid, high throughput, and the cost is acceptable while it needs specialized microarray facilities. For complete details on the use and execution of this protocol, please refer to Li et al. (2020, 2021a, 2021b).
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Peptide Fragments / Epitopes, B-Lymphocyte / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral / Antigens, Viral Topics: Vaccines Limits: Humans Language: English Journal: STAR Protoc Year: 2021 Document Type: Article Affiliation country: J.xpro.2021.100707

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Peptide Fragments / Epitopes, B-Lymphocyte / Spike Glycoprotein, Coronavirus / SARS-CoV-2 / COVID-19 / Antibodies, Viral / Antigens, Viral Topics: Vaccines Limits: Humans Language: English Journal: STAR Protoc Year: 2021 Document Type: Article Affiliation country: J.xpro.2021.100707