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Effect of different storage conditions on COVID-19 RT-PCR results.
Yilmaz Gulec, Elif; Cesur, Nevra P; Yesilyurt Fazlioglu, Gonca; Kazezoglu, Cemal.
  • Yilmaz Gulec E; Department of Medical Genetics, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.
  • Cesur NP; Department of COVID-19 Diagnostic Center, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.
  • Yesilyurt Fazlioglu G; Department of COVID-19 Diagnostic Center, TUSEB, Turkey Institutes of Health COVID-19 Diagnostic Center, Istanbul Kanuni Sultan Suleyman Training and Research Hospital, Health Sciences University, Istanbul, Turkey.
  • Kazezoglu C; Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Yildiz Technical University, Istanbul, Turkey.
J Med Virol ; 93(12): 6575-6581, 2021 12.
Article in English | MEDLINE | ID: covidwho-1530180
ABSTRACT
Reliable and rapid detection of severe acute respiratory syndrome coronavirus 2 in laboratory setting is critical to control the pandemic. We aimed to an evaluated polymerase chain reaction (PCR) efficiency of nasopharyngeal swabs stored in viral transport medium (VTM) in different temperatures. Ninety swabs taken into VTM were analyzed at the first hour, then divided into two groups with similar numbers of positive and negative samples. Positive samples of each group were also subgrouped according to Fam CT values as low CT (<25), medium CT (25-32), and high CT (32-38) groups. One group was stored at 4°C, while the other was stored at room temperature, PCR analyses were repeated every 24 h for 5 days and on Day 12. There was a total of 30 positive samples (12 low CT, 11 medium CT, and 7 high CT). The CT values of both groups remained unchanged in first 3 days while the CT values of the room temperature group increased after the third day. All of the positive samples remained positive in both groups for the first 5 days. On the 12th day, the total number of positives decreased to 8 in the room temperature group and 11 in the 4°C groups. All the low CT samples remained positive in both groups. In conclusion, it is safe to store positive samples in room temperature for up to 5 days. Only samples with high viral loads remain positive for 12 days, regardless of whether stored at room temperature or 4°C. Negative samples don't turn to invalid if stored in VTM.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Specimen Handling / Real-Time Polymerase Chain Reaction / COVID-19 Testing Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.27204

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Specimen Handling / Real-Time Polymerase Chain Reaction / COVID-19 Testing Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Limits: Humans Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.27204