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MiRNA Profiling in Plasma and Placenta of SARS-CoV-2-Infected Pregnant Women.
Saulle, Irma; Garziano, Micaela; Fenizia, Claudio; Cappelletti, Gioia; Parisi, Francesca; Clerici, Mario; Cetin, Irene; Savasi, Valeria; Biasin, Mara.
  • Saulle I; Department of Biomedical and Clinical Sciences, University of Milan, 20157 Milan, Italy.
  • Garziano M; Department of Pathophysiology and Transplantation, University of Milan, 20122 Milan, Italy.
  • Fenizia C; Department of Biomedical and Clinical Sciences, University of Milan, 20157 Milan, Italy.
  • Cappelletti G; Department of Pathophysiology and Transplantation, University of Milan, 20122 Milan, Italy.
  • Parisi F; Department of Biomedical and Clinical Sciences, University of Milan, 20157 Milan, Italy.
  • Clerici M; Department of Pathophysiology and Transplantation, University of Milan, 20122 Milan, Italy.
  • Cetin I; Department of Biomedical and Clinical Sciences, University of Milan, 20157 Milan, Italy.
  • Savasi V; Unit of Obstetrics and Gynecology, ASST Fatebenefratelli-Sacco, Department of Biological and Clinical Sciences L. Sacco, University of Milan, 20157 Milan, Italy.
  • Biasin M; Department of Pathophysiology and Transplantation, University of Milan, 20122 Milan, Italy.
Cells ; 10(7)2021 07 15.
Article in English | MEDLINE | ID: covidwho-1314589
ABSTRACT
MicroRNAs are gene expression regulators associated with several human pathologies, including those generated by viral infections. Their role in SARS-CoV-2 infection and COVID-19 has been investigated and reviewed in many informative studies; however, a thorough miRNA outline in SARS-CoV-2-infected pregnant women (SIPW), at both systemic and placental levels, is missing. To fill this gap, blood and placenta biopsies collected at delivery from 15 asymptomatic SIPW were immediately analysed for miRNA expression (n = 84) (QPCR array), antiviral/immune mRNA target expression (n = 74) (QGene) and cytokine/chemokines production (n = 27) (Multiplex ELISA). By comparing these results with those obtained from six uninfected pregnant women (UPW), we observed that, following SARS-CoV-2 infection, the transcriptomic profile of pregnant women is significantly altered in different anatomical districts, even in the absence of clinical symptoms and vertical transmission. This characteristic combination of miRNA and antiviral/immune factors seems to control both the infection and the dysfunctional immune reaction, thus representing a positive correlate of protection and a potential therapeutic target against SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pregnancy Complications, Infectious / MicroRNAs / COVID-19 Type of study: Diagnostic study Limits: Adult / Female / Humans / Pregnancy / Young adult Language: English Year: 2021 Document Type: Article Affiliation country: Cells10071788

Full text: Available Collection: International databases Database: MEDLINE Main subject: Pregnancy Complications, Infectious / MicroRNAs / COVID-19 Type of study: Diagnostic study Limits: Adult / Female / Humans / Pregnancy / Young adult Language: English Year: 2021 Document Type: Article Affiliation country: Cells10071788