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Dynamic Assay for Profiling Anti-SARS-CoV-2 Antibodies and Their ACE2/Spike RBD Neutralization Capacity.
Phelan, Thomas; Dunne, Jean; Conlon, Niall; Cheallaigh, Clíona Ní; Abbott, W Mark; Faba-Rodriguez, Raquel; Amanat, Fatima; Krammer, Florian; Little, Mark A; Hughes, Gerry; Bergin, Colm; Kerr, Colm; Sundaresan, Sudharshana; Long, Aideen; McCormack, William; Brady, Gareth.
  • Phelan T; Department of Clinical Medicine, Trinity Translational Medicine Institute, School of Medicine, Trinity College Dublin, D08 W9RT Dublin, Ireland.
  • Dunne J; Department of Immunology, St. James's Hospital, D08 NHY1 Dublin, Ireland.
  • Conlon N; Department of Immunology, St. James's Hospital, D08 NHY1 Dublin, Ireland.
  • Cheallaigh CN; Department of Immunology, School of Medicine, Trinity College Dublin, D02 R590 Dublin, Ireland.
  • Abbott WM; Department of Clinical Medicine, Trinity Translational Medicine Institute, School of Medicine, Trinity College Dublin, D08 W9RT Dublin, Ireland.
  • Faba-Rodriguez R; Department of Infectious Diseases, St James's Hospital, D08 NHY1 Dublin, Ireland.
  • Amanat F; Peak Proteins Ltd., Alderley Park, Mereside, Macclesfield SK10 4TG, UK.
  • Krammer F; Peak Proteins Ltd., Alderley Park, Mereside, Macclesfield SK10 4TG, UK.
  • Little MA; Department of Microbiology, Icahn School of Medicine, Mount Sinai, New York, NY 10029-5674, USA.
  • Hughes G; Department of Microbiology, Icahn School of Medicine, Mount Sinai, New York, NY 10029-5674, USA.
  • Bergin C; Trinity Health Kidney Centre, Trinity Translational Medicine Institute, Trinity College Dublin, St. James' Hospital Campus, D08 W9RT Dublin, Ireland.
  • Kerr C; Department of Clinical Medicine, Trinity Translational Medicine Institute, School of Medicine, Trinity College Dublin, D08 W9RT Dublin, Ireland.
  • Sundaresan S; Department of Pharmacy, St James's Hospital, D08 NHY1 Dublin, Ireland.
  • Long A; Department of Clinical Medicine, Trinity Translational Medicine Institute, School of Medicine, Trinity College Dublin, D08 W9RT Dublin, Ireland.
  • McCormack W; Department of Infectious Diseases, St James's Hospital, D08 NHY1 Dublin, Ireland.
  • Brady G; Department of Clinical Medicine, Trinity Translational Medicine Institute, School of Medicine, Trinity College Dublin, D08 W9RT Dublin, Ireland.
Viruses ; 13(7)2021 07 15.
Article in English | MEDLINE | ID: covidwho-1314763
ABSTRACT
Serological assays have been widely employed during the coronavirus disease 2019 (COVID-19) pandemic to measure antibody responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to track seroconversion in populations. However, currently available assays do not allow determination of neutralization capacity within the assay protocol. Furthermore, commercial serology assays have a high buy-in cost that is inaccessible for many research groups. We have replicated the serological enzyme-linked immunosorbent assay for the detection of SARS-CoV-2 antibody isotypes, developed at the Icahn School of Medicine at Mount Sinai, New York. Additionally, we have modified the protocol to include a neutralization assay with only a minor modification to this protocol. We used this assay to screen local COVID-19 patient sera (n = 91) and pre-COVID-19 control sera (n = 103), and obtained approximate parity with approved commercial anti-nucleoprotein-based assays with these sera. Furthermore, data from our neutralization assay closely aligns with that generated using a spike-based pseudovirus infection model when a subset of patient sera was analyzed.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Angiotensin-Converting Enzyme 2 / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Observational study / Randomized controlled trials Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: V13071371

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antibodies, Neutralizing / Spike Glycoprotein, Coronavirus / Angiotensin-Converting Enzyme 2 / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Experimental Studies / Observational study / Randomized controlled trials Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: V13071371