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RBD-Modified Bacterial Vesicles Elicited Potential Protective Immunity against SARS-CoV-2.
Yang, Zhongqian; Hua, Liangqun; Yang, Mengli; Liu, Shu-Qun; Shen, Jianxin; Li, Weiran; Long, Qiong; Bai, Hongmei; Yang, Xu; Ren, Zhaoling; Zheng, Xiao; Sun, Wenjia; Ye, Chao; Li, Duo; Zheng, Peng; He, Jinrong; Chen, Yongjun; Huang, Weiwei; Peng, Xiaozhong; Ma, Yanbing.
  • Yang Z; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Hua L; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Yang M; Yunnan University, Kunming, China.
  • Liu SQ; National Kunming High-level Biosafety Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Shen J; Yunnan University, Kunming, China.
  • Li W; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan & School of Life Sciences, Yunnan University, Kunming, China.
  • Long Q; Yunnan University, Kunming, China.
  • Bai H; State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan & School of Life Sciences, Yunnan University, Kunming, China.
  • Yang X; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Ren Z; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Zheng X; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Sun W; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Ye C; The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Li D; Kunming Medical University, Kunming, China.
  • Zheng P; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • He J; Yunnan University, Kunming, China.
  • Chen Y; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Huang W; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Peng X; Laboratory of Molecular Immunology, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
  • Ma Y; Department of Acute Infectious Diseases Control and Prevention, Yunnan Provincial Center for Disease Control and Prevention, Kunming, China.
Nano Lett ; 21(14): 5920-5930, 2021 07 28.
Article in English | MEDLINE | ID: covidwho-1316697
ABSTRACT
The disease caused by SARS-CoV-2 infection threatens human health. In this study, we used high-pressure homogenization technology not only to efficiently drive the bacterial membrane to produce artificial vesicles but also to force the fusion protein ClyA-receptor binding domain (RBD) to pass through gaps in the bacterial membrane to increase the contact between ClyA-RBD and the membrane. Therefore, the load of ClyA-RBD on the membrane is substantially increased. Using this technology, we constructed a "ring-like" bacterial biomimetic vesicle (BBV) loaded with polymerized RBD (RBD-BBV). RBD-BBVs injected subcutaneously can accumulate in lymph nodes, promote antigen uptake and processing, and elicit SARS-CoV-2-specific humoral and cellular immune responses in mice. In conclusion, we evaluated the potential of this novel bacterial vesicle as a vaccine delivery system and provided a new idea for the development of SARS-CoV-2 vaccines.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Journal: Nano Lett Year: 2021 Document Type: Article Affiliation country: Acs.nanolett.1c00680

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Experimental Studies Topics: Vaccines Limits: Animals / Humans Language: English Journal: Nano Lett Year: 2021 Document Type: Article Affiliation country: Acs.nanolett.1c00680