Exponential Amplification Using Photoredox Autocatalysis.
J Am Chem Soc
; 143(30): 11544-11553, 2021 08 04.
Article
in English
| MEDLINE | ID: covidwho-1319014
ABSTRACT
Exponential molecular amplification such as the polymerase chain reaction is a powerful tool that allows ultrasensitive biodetection. Here, we report a new exponential amplification strategy based on photoredox autocatalysis, where eosin Y, a photocatalyst, amplifies itself by activating a nonfluorescent eosin Y derivative (EYH3-) under green light. The deactivated photocatalyst is stable and rapidly activated under low-intensity light, making the eosin Y amplification suitable for resource-limited settings. Through steady-state kinetic studies and reaction modeling, we found that EYH3- is either oxidized to eosin Y via one-electron oxidation by triplet eosin Y and subsequent 1e-/H+ transfer, or activated by singlet oxygen with the risk of degradation. By reducing the rate of the EYH3- degradation, we successfully improved EYH3--to-eosin Y recovery, achieving efficient autocatalytic eosin Y amplification. Additionally, to demonstrate its flexibility in output signals, we coupled the eosin Y amplification with photoinduced chromogenic polymerization, enabling sensitive visual detection of analytes. Finally, we applied the exponential amplification methods in developing bioassays for detection of biomarkers including SARS-CoV-2 nucleocapsid protein, an antigen used in the diagnosis of COVID-19.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Spectrometry, Fluorescence
/
Eosine Yellowish-(YS)
/
Coronavirus Nucleocapsid Proteins
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
J Am Chem Soc
Year:
2021
Document Type:
Article
Affiliation country:
Jacs.1c04236
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