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Development of spike protein-based fluorescence lateral flow assay for the simultaneous detection of SARS-CoV-2 specific IgM and IgG.
Wang, Chongwen; Shi, Dawei; Wan, Nan; Yang, Xingsheng; Liu, Haifeng; Gao, Huixia; Zhang, Minli; Bai, Zikun; Li, Dingchen; Dai, Erhei; Rong, Zhen; Wang, Shengqi.
  • Wang C; Beijing Institute of Radiation Medicine, Beijing 100850, PR China. sqwang@bmi.ac.cn and College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China.
  • Shi D; National Institutes for Food and Drug Control, Beijing 100050, PR China.
  • Wan N; The General Hospital of Northern Theater Command, Shenyang 110016, PR China.
  • Yang X; Beijing Institute of Radiation Medicine, Beijing 100850, PR China. sqwang@bmi.ac.cn and College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China.
  • Liu H; Beijing Institute of Radiation Medicine, Beijing 100850, PR China. sqwang@bmi.ac.cn and College of Life Sciences, Anhui Agricultural University, Hefei 230036, PR China.
  • Gao H; Division of Liver Diseases, The Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang 050021, China. daieh2008@126.com.
  • Zhang M; Beijing Institute of Radiation Medicine, Beijing 100850, PR China. sqwang@bmi.ac.cn.
  • Bai Z; Beijing Institute of Radiation Medicine, Beijing 100850, PR China. sqwang@bmi.ac.cn and Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Beijing 100850, PR China. rongzhen0525@sina.com.
  • Li D; The General Hospital of Northern Theater Command, Shenyang 110016, PR China.
  • Dai E; Division of Liver Diseases, The Fifth Hospital of Shijiazhuang, Hebei Medical University, Shijiazhuang 050021, China. daieh2008@126.com.
  • Rong Z; Beijing Institute of Radiation Medicine, Beijing 100850, PR China. sqwang@bmi.ac.cn and Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Beijing 100850, PR China. rongzhen0525@sina.com.
  • Wang S; Beijing Institute of Radiation Medicine, Beijing 100850, PR China. sqwang@bmi.ac.cn and Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Disease, Beijing 100850, PR China. rongzhen0525@sina.com.
Analyst ; 146(12): 3908-3917, 2021 Jun 14.
Article in English | MEDLINE | ID: covidwho-1319050
Semantic information from SemMedBD (by NLM)
1. Assay USES M Protei
Subject
Assay
Predicate
USES
Object
M Protei
2. Detection USES M Protei
Subject
Detection
Predicate
USES
Object
M Protei
3. 2019 novel coronavirus CAUSES Disease
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
Disease
4. Infected PROCESS_OF Persons
Subject
Infected
Predicate
PROCESS_OF
Object
Persons
5. Serum PART_OF Homo sapiens
Subject
Serum
Predicate
PART_OF
Object
Homo sapiens
6. Serum LOCATION_OF SARS-CoV-2 IgM|CD40LG
Subject
Serum
Predicate
LOCATION_OF
Object
SARS-CoV-2 IgM|CD40LG
7. Respiration Disorders PROCESS_OF Patients
Subject
Respiration Disorders
Predicate
PROCESS_OF
Object
Patients
8. Assay USES M Protein, multiple myeloma
Subject
Assay
Predicate
USES
Object
M Protein, multiple myeloma
9. Detection USES M Protein, multiple myeloma
Subject
Detection
Predicate
USES
Object
M Protein, multiple myeloma
10. 2019 novel coronavirus CAUSES Disease
Subject
2019 novel coronavirus
Predicate
CAUSES
Object
Disease
11. Infected PROCESS_OF Persons
Subject
Infected
Predicate
PROCESS_OF
Object
Persons
12. Serum PART_OF Homo sapiens
Subject
Serum
Predicate
PART_OF
Object
Homo sapiens
13. Serum LOCATION_OF SARS-CoV-2 IgM|CD40LG
Subject
Serum
Predicate
LOCATION_OF
Object
SARS-CoV-2 IgM|CD40LG
14. Respiration Disorders PROCESS_OF Patients
Subject
Respiration Disorders
Predicate
PROCESS_OF
Object
Patients
ABSTRACT
The pandemic outbreak of the 2019 coronavirus disease (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is still spreading rapidly and poses a great threat to human health. As such, developing rapid and accurate immunodiagnostic methods for the identification of infected persons is needed. Here, we proposed a simple but sensitive on-site testing method based on spike protein-conjugated quantum dot (QD) nanotag-integrated lateral flow immunoassay (LFA) to simultaneously detect SARS-CoV-2-specific IgM and IgG in human serum. Advanced silica-core@dual QD-shell nanocomposites (SiO2@DQD) with superior luminescence and stability were prepared to serve as fluorescent nanotags in the LFA strip and guarantee high sensitivity and reliability of the assay. The performance of the SiO2@DQD-strip was fully optimized and confirmed by using 10 positive serum samples from COVID-19 patients and 10 negative samples from patients with other respiratory diseases. The practical clinical value of the assay was further evaluated by testing 316 serum samples (114 positive and 202 negative samples). The overall detection sensitivity and specificity reached 97.37% (111/114) and 95.54% (193/202), respectively, indicating the huge potential of our proposed method for the rapid and accurate detection of SARS-CoV-2-infected persons and asymptomatic carriers.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Analyst Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Analyst Year: 2021 Document Type: Article