Pre-analytical sample handling effects on blood cytokine levels: quality control of a COVID-19 biobank.
Biomark Med
; 15(12): 987-997, 2021 08.
Article
in English
| MEDLINE | ID: covidwho-1320567
ABSTRACT
Aim:
We investigated the effect of pre-analytical sample handling variations on coronavirus disease 2019-relevant circulating cytokine levels IFN-γ, IL-10, IL-12p70, IL-17A, IL-6 and TNF-α. Materials &methods:
We collected blood in different collection tubes (ethylenediaminetetraacetic acid, sodium citrate, lithium heparin, serum), and subjected ethylenediaminetetraacetic acid plasma to among others increasing delays in centrifugation or -80°C storage. Six subjects were included in each experimental condition. Cytokine levels were measured in these samples using the Simoa Cytokine 6-plex kit.Results:
Different tube types resulted in different blood cytokine levels. IL-17A and IL-6 levels declined with 3 h centrifugation delay. IFN-γ levels declined with 24 h postcentrifugation storage delay. IL-17A levels declined with 2-week storage delay.Conclusion:
It is recommended to centrifuge tubes quickly following collection, for accurate cytokine measurement.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Quality Control
/
Specimen Handling
/
Cytokines
/
Biological Specimen Banks
/
SARS-CoV-2
/
COVID-19
Type of study:
Experimental Studies
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
English
Journal:
Biomark Med
Journal subject:
Biochemistry
/
Medicine
Year:
2021
Document Type:
Article
Affiliation country:
Bmm-2020-0770
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