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Liposome-mediated detection of SARS-CoV-2 RNA-positive extracellular vesicles in plasma.
Ning, Bo; Huang, Zhen; Youngquist, Brady M; Scott, John W; Niu, Alex; Bojanowski, Christine M; Zwezdaryk, Kevin J; Saba, Nakhle S; Fan, Jia; Yin, Xiao-Ming; Cao, Jing; Lyon, Christopher J; Li, Chen-Zhong; Roy, Chad J; Hu, Tony Y.
  • Ning B; Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA. bning1@tulane.edu.
  • Huang Z; Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, LA, USA. bning1@tulane.edu.
  • Youngquist BM; Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
  • Scott JW; Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
  • Niu A; Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
  • Bojanowski CM; Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
  • Zwezdaryk KJ; Department of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
  • Saba NS; Section of Hematology and Medical Oncology, Tulane University School of Medicine, New Orleans, LA, USA.
  • Fan J; Section of Pulmonary Diseases, Tulane University School of Medicine, New Orleans, LA, USA.
  • Yin XM; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA, USA.
  • Cao J; Section of Hematology and Medical Oncology, Tulane University School of Medicine, New Orleans, LA, USA.
  • Lyon CJ; Center for Cellular and Molecular Diagnostics, Tulane University School of Medicine, New Orleans, LA, USA.
  • Li CZ; Department of Biochemistry and Molecular Biology, Tulane University School of Medicine, New Orleans, LA, USA.
  • Roy CJ; Department of Pathology and Laboratory Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
  • Hu TY; Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Nat Nanotechnol ; 16(9): 1039-1044, 2021 09.
Article in English | MEDLINE | ID: covidwho-1322483
ABSTRACT
Plasma SARS-CoV-2 RNA may represent a viable diagnostic alternative to respiratory RNA levels, which rapidly decline after infection. Quantitative PCR with reverse transcription (RT-qPCR) reference assays exhibit poor performance with plasma, probably reflecting the dilution and degradation of viral RNA released into the circulation, but these issues could be addressed by analysing viral RNA packaged into extracellular vesicles. Here we describe an assay approach in which extracellular vesicles directly captured from plasma are fused with reagent-loaded liposomes to sensitively amplify and detect a SARS-CoV-2 gene target. This approach accurately identified patients with COVID-19, including challenging cases missed by RT-qPCR. SARS-CoV-2-positive extracellular vesicles were detected at day 1 post-infection, and plateaued from day 6 to the day 28 endpoint in a non-human primate model, while signal durations for 20-60 days were observed in young children. This nanotechnology approach uses a non-infectious sample and extends virus detection windows, offering a tool to support COVID-19 diagnosis in patients without SARS-CoV-2 RNA detectable in the respiratory tract.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Extracellular Vesicles / SARS-CoV-2 / COVID-19 / Liposomes Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Nat Nanotechnol Year: 2021 Document Type: Article Affiliation country: S41565-021-00939-8

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / Extracellular Vesicles / SARS-CoV-2 / COVID-19 / Liposomes Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Nat Nanotechnol Year: 2021 Document Type: Article Affiliation country: S41565-021-00939-8