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Gold-Oligonucleotide Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences.
Chauhan, Veeren M; Elsutohy, Mohamed M; McClure, C Patrick; Irving, William L; Roddis, Neil; Aylott, Jonathan W.
  • Chauhan VM; School of Pharmacy, Boots Science Building, University of Nottingham, Nottingham NG7 2RD, UK.
  • Elsutohy MM; TBG Solutions Ltd. 3A Midland Court, Barlborough Links, Barlborough, Chesterfield, Derbyshire S43 4UL, UK.
  • McClure CP; Schulich School of Engineering, University of Calgary, Calgary, AB T2N 4V8, Canada.
  • Irving WL; Queen's Medical Centre, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.
  • Roddis N; Queen's Medical Centre, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.
  • Aylott JW; TBG Solutions Ltd. 3A Midland Court, Barlborough Links, Barlborough, Chesterfield, Derbyshire S43 4UL, UK.
Biosensors (Basel) ; 11(7)2021 Jul 14.
Article in English | MEDLINE | ID: covidwho-1323111
ABSTRACT
Enteroviruses are ubiquitous mammalian pathogens that can produce mild to life-threatening disease. We developed a multimodal, rapid, accurate and economical point-of-care biosensor that can detect nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and oligonucleotides to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral nucleic acid sequence (23 bases), which was identified through in silico screening. Oligonucleotides were designed to demonstrate specific complementarity towards the target enteroviral nucleic acid to produce aggregated gold-oligonucleotide nanoconstructs. The conserved target enteroviral nucleic acid sequence (≥1 × 10-7 M, ≥1.4 × 10-14 g/mL) initiates gold-oligonucleotide nanoconstruct disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow assays that utilise gold-oligonucleotide nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (<60 s), and could be interpreted with a bespoke software and hardware electronic interface. We anticipate that our methodology will translate in silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave the way forward in the clinical evaluation of disease and complement existing strategies to overcome antimicrobial resistance.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acids / Biosensing Techniques / Metal Nanoparticles Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Bios11070238

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Nucleic Acids / Biosensing Techniques / Metal Nanoparticles Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: Bios11070238