Your browser doesn't support javascript.
Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples.
Kaku, Norihito; Hashiguchi, Kohji; Akamatsu, Norihiko; Wakigawa, Fumiko; Matsuda, Junichi; Komaru, Kenzo; Nakao, Takumi; Harada, Yosuke; Hara, Atsuko; Uno, Naoki; Sakamoto, Kei; Morinaga, Yoshitomo; Kitazaki, Takeshi; Hasegawa, Hiroo; Miyazaki, Taiga; Fukuda, Masaaki; Izumikawa, Koichi; Mukae, Hiroshi; Yanagihara, Katsunori.
  • Kaku N; Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan. kaku-ngs@umin.ac.jp.
  • Hashiguchi K; Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan. kaku-ngs@umin.ac.jp.
  • Akamatsu N; Department of Respiratory Medicine, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan.
  • Wakigawa F; Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.
  • Matsuda J; Department of Clinical Laboratory, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan.
  • Komaru K; Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.
  • Nakao T; Department of Clinical Laboratory, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan.
  • Harada Y; Department of Respiratory Medicine, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan.
  • Hara A; Department of Respiratory Medicine, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan.
  • Uno N; Department of Respiratory Medicine, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan.
  • Sakamoto K; Department of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
  • Morinaga Y; Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
  • Kitazaki T; Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.
  • Hasegawa H; Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
  • Miyazaki T; Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.
  • Fukuda M; Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
  • Izumikawa K; Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.
  • Mukae H; Department of Respiratory Medicine, Japanese Red Cross Nagasaki Genbaku Hospital, Nagasaki, Japan.
  • Yanagihara K; Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Eur J Clin Microbiol Infect Dis ; 40(8): 1743-1748, 2021 Aug.
Article in English | MEDLINE | ID: covidwho-1323942
ABSTRACT
We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza A virus / Influenza B virus / Nasopharynx / Influenza, Human Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Eur J Clin Microbiol Infect Dis Journal subject: Communicable Diseases / Microbiology Year: 2021 Document Type: Article Affiliation country: S10096-021-04193-7

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Main subject: Influenza A virus / Influenza B virus / Nasopharynx / Influenza, Human Type of study: Cohort study / Diagnostic study / Experimental Studies / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Eur J Clin Microbiol Infect Dis Journal subject: Communicable Diseases / Microbiology Year: 2021 Document Type: Article Affiliation country: S10096-021-04193-7