CRISPR-based diagnostics.
Nat Biomed Eng
; 5(7): 643-656, 2021 07.
Article
in English
| MEDLINE | ID: covidwho-1324420
ABSTRACT
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic acids are among the most sensitive and specific, yet most such assays require costly equipment and trained personnel. Recent developments in diagnostic technologies, in particular those leveraging clustered regularly interspaced short palindromic repeats (CRISPR), aim to enable accurate testing at home, at the point of care and in the field. In this Review, we provide a rundown of the rapidly expanding toolbox for CRISPR-based diagnostics, in particular the various assays, preamplification strategies and readouts, and highlight their main applications in the sensing of a wide range of molecular targets relevant to human health.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Nucleic Acids
/
Communicable Diseases
/
Nucleic Acid Amplification Techniques
/
CRISPR-Cas Systems
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Nat Biomed Eng
Year:
2021
Document Type:
Article
Affiliation country:
S41551-021-00760-7
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