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Vapourized hydrogen peroxide decontamination in a hospital setting inactivates SARS-CoV-2 and HCoV-229E without compromising filtration efficiency of unexpired N95 respirators.
Christie-Holmes, Natasha; Tyli, Rachel; Budylowski, Patrick; Guvenc, Furkan; Weiner, Amit; Poon, Betty; Speck, Mary; Naugler, Stephenie; Rainville, Allen; Ghalami, Ayoob; McCaw, Shannon; Hayes, Steven; Mubareka, Samira; Gray-Owen, Scott D; Rotstein, Ori D; Kandel, Rita A; Scott, James A.
  • Christie-Holmes N; Combined Containment Level 3 Unit, Temerty Faculty of Medicine, University of Toronto, Toronto, ON Canada M5S 1A8.
  • Tyli R; Dalla Lana School of Public Health, University of Toronto, 223 College Street, Toronto, ON, Canada, M5T 1R4.
  • Budylowski P; Combined Containment Level 3 Unit, Temerty Faculty of Medicine, University of Toronto, Toronto, ON Canada M5S 1A8; Institute of Medicine, Temerty Faculty of Medicine, University of Toronto, 1 King's College Circle, Toronto, ON, Canada, M5S 1A8.
  • Guvenc F; Combined Containment Level 3 Unit, Temerty Faculty of Medicine, University of Toronto, Toronto, ON Canada M5S 1A8; Department of Molecular Genetics, Temerty Faculty of Medicine, University of Toronto, 1 King's College Circle, Toronto, ON Canada M5S 1A8.
  • Weiner A; Combined Containment Level 3 Unit, Temerty Faculty of Medicine, University of Toronto, Toronto, ON Canada M5S 1A8; Department of Molecular Genetics, Temerty Faculty of Medicine, University of Toronto, 1 King's College Circle, Toronto, ON Canada M5S 1A8.
  • Poon B; Combined Containment Level 3 Unit, Temerty Faculty of Medicine, University of Toronto, Toronto, ON Canada M5S 1A8.
  • Speck M; Dalla Lana School of Public Health, University of Toronto, 223 College Street, Toronto, ON, Canada, M5T 1R4.
  • Naugler S; Unity Health Toronto, 30 Bond St, Toronto, ON Canada M5B 1W8.
  • Rainville A; Unity Health Toronto, 30 Bond St, Toronto, ON Canada M5B 1W8.
  • Ghalami A; Office of Environmental Health & Safety, University of Toronto, 215 Huron St, Toronto, ON Canada M5S 1A2.
  • McCaw S; Office of Environmental Health & Safety, University of Toronto, 215 Huron St, Toronto, ON Canada M5S 1A2.
  • Hayes S; Unity Health Toronto, 30 Bond St, Toronto, ON Canada M5B 1W8.
  • Mubareka S; Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 1 King's College Cir, Toronto, ON, Canada, M5S 1A8; Sunnybrook Health Sciences Centre, 2075 Bayview Av, Toronto, ON Canada M4N 3M5.
  • Gray-Owen SD; Combined Containment Level 3 Unit, Temerty Faculty of Medicine, University of Toronto, Toronto, ON Canada M5S 1A8; Department of Molecular Genetics, Temerty Faculty of Medicine, University of Toronto, 1 King's College Circle, Toronto, ON Canada M5S 1A8.
  • Rotstein OD; Unity Health Toronto, 30 Bond St, Toronto, ON Canada M5B 1W8.
  • Kandel RA; Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 1 King's College Cir, Toronto, ON, Canada, M5S 1A8.
  • Scott JA; Dalla Lana School of Public Health, University of Toronto, 223 College Street, Toronto, ON, Canada, M5T 1R4; Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 1 King's College Cir, Toronto, ON, Canada, M5S 1A8. Electronic address: james.scott@uto
Am J Infect Control ; 49(10): 1227-1231, 2021 10.
Article in English | MEDLINE | ID: covidwho-1328688
ABSTRACT

BACKGROUND:

The COVID-19 pandemic highlighted the need for evidence-based approaches to decontamination and reuse of N95 filtering facepiece respirators (FFRs). We sought to determine whether vapourized hydrogen peroxide (VHP) reduced SARS-CoV-2 bioburden on FFRs without compromising filtration efficiency. We also investigated coronavirus HCoV-229E as a surrogate for decontamination validation testing.

METHODS:

N95 FFRs were laced with SARS-CoV-2 or HCoV-229E and treated with VHP in a hospital reprocessing facility. After sterilization, viral burden was determined using viral outgrowth in a titration assay, and filtration efficiency of FFRs was tested against ATSM F2299 and NIOSH TEB-STP-APR-0059.

RESULTS:

Viable SARS-CoV-2 virus was not detected after VHP treatment. One replicate of the HCoV-229E laced FFRs yielded virus after processing. Unexpired N95 FFRs retained full filtration efficiency after VHP processing. Expired FFRs failed to meet design-specified filtration efficiency and therefore are unsuitable for reprocessing.

DISCUSSION:

In-hospital VHP is an effective decontaminant for SARS-CoV-2 on FFRs. Further, filtration efficiency of unexpired respirators is not affected by this decontamination process.

CONCLUSIONS:

VHP is effective in inactivating SARS-CoV-2 on FFRs without compromising filtration efficiency. HCoV-229E is a suitable surrogate for SARS-CoV-2 for disinfection studies.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus 229E, Human / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Am J Infect Control Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus 229E, Human / COVID-19 Type of study: Prognostic study Limits: Humans Language: English Journal: Am J Infect Control Year: 2021 Document Type: Article