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Point-of-need detection of pathogen-specific nucleic acid targets using magnetic particle spectroscopy.
Rösch, Enja Laureen; Zhong, Jing; Lak, Aidin; Liu, Zhe; Etzkorn, Markus; Schilling, Meinhard; Ludwig, Frank; Viereck, Thilo; Lalkens, Birka.
  • Rösch EL; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
  • Zhong J; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
  • Lak A; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
  • Liu Z; Institute of Applied Physics, TU Braunschweig, Mendelssohnstraße 2, 38106, Braunschweig, Germany.
  • Etzkorn M; Institute of Applied Physics, TU Braunschweig, Mendelssohnstraße 2, 38106, Braunschweig, Germany.
  • Schilling M; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
  • Ludwig F; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
  • Viereck T; Institute for Electrical Measurement Science and Fundamental Electrical Engineering and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Hans-Sommer-Str. 66, Braunschweig, 38106, Germany.
  • Lalkens B; Institute of Semiconductor Technology and Laboratory for Emerging Nanometrology (LENA), TU Braunschweig, Langer Kamp 6a/b, 38106, Braunschweig, Germany. Electronic address: b.lalkens@tu-braunschweig.de.
Biosens Bioelectron ; 192: 113536, 2021 Nov 15.
Article in English | MEDLINE | ID: covidwho-1330665
ABSTRACT
The ongoing COVID-19 pandemic stresses the need for widely available diagnostic tests for the presence of SARS-CoV-2 in individuals. Due to the limited availability of vaccines, diagnostic assays which are cheap, easy-to-use at the point-of-need, reliable and fast, are currently the only way to control the pandemic situation. Here we present a diagnostic assay for the detection of pathogen-specific nucleic acids based on changes of the magnetic response of magnetic nanoparticles The target-mediated hybridization of modified nanoparticles leads to an increase in the hydrodynamic radius. This resulting change in the magnetic behaviour in an ac magnetic field can be measured via magnetic particle spectroscopy (MPS), providing a viable tool for the accurate detection of target nucleic acids. In this work we show that single stranded DNA can be detected in a concentration-dependent manner by these means. In addition to detecting synthetic DNA with an arbitrary sequence in a concentration down to 500 pM, we show that RNA and SARS-CoV-2-specific DNA as well as saliva as a sample medium can be used for an accurate assay. These proof-of-principle experiments show the potential of MPS based assays for the reliable and fast diagnostics of pathogens like SARS-CoV-2 in a point-of-need fashion without the need of complex sample preparation.
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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Nucleic Acids / Biosensing Techniques / COVID-19 Subject: Nucleic Acids / Biosensing Techniques / COVID-19 Type of study: Diagnostic study Language: English Journal: Biosens Bioelectron Clinical aspect: Diagnosis Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Nucleic Acids / Biosensing Techniques / COVID-19 Subject: Nucleic Acids / Biosensing Techniques / COVID-19 Type of study: Diagnostic study Language: English Journal: Biosens Bioelectron Clinical aspect: Diagnosis Year: 2021
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