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Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology.
John, Goldin; Sahajpal, Nikhil Shri; Mondal, Ashis K; Ananth, Sudha; Williams, Colin; Chaubey, Alka; Rojiani, Amyn M; Kolhe, Ravindra.
  • John G; Department of Pathology, Medical College of Georgia, Augusta University, BAE 2576, 1120 15th Street, Augusta, GA 30912, USA.
  • Sahajpal NS; Department of Pathology, Medical College of Georgia, Augusta University, BAE 2576, 1120 15th Street, Augusta, GA 30912, USA.
  • Mondal AK; Department of Pathology, Medical College of Georgia, Augusta University, BAE 2576, 1120 15th Street, Augusta, GA 30912, USA.
  • Ananth S; Department of Pathology, Medical College of Georgia, Augusta University, BAE 2576, 1120 15th Street, Augusta, GA 30912, USA.
  • Williams C; Department of Pathology, Medical College of Georgia, Augusta University, BAE 2576, 1120 15th Street, Augusta, GA 30912, USA.
  • Chaubey A; Department of Pathology, Medical College of Georgia, Augusta University, BAE 2576, 1120 15th Street, Augusta, GA 30912, USA.
  • Rojiani AM; Bionano Genomics Inc., San Diego, CA 92121, USA.
  • Kolhe R; Department of Pathology, Penn State University College of Medicine, Hershey, PA 16802, USA.
Curr Issues Mol Biol ; 43(2): 845-867, 2021 Jul 30.
Article in English | MEDLINE | ID: covidwho-1335016
ABSTRACT
This review discusses the current testing methodologies for COVID-19 diagnosis and explores next-generation sequencing (NGS) technology for the detection of SARS-CoV-2 and monitoring phylogenetic evolution in the current COVID-19 pandemic. The review addresses the development, fundamentals, assay quality control and bioinformatics processing of the NGS data. This article provides a comprehensive review of the obstacles and opportunities facing the application of NGS technologies for the diagnosis, surveillance, and study of SARS-CoV-2 and other infectious diseases. Further, we have contemplated the opportunities and challenges inherent in the adoption of NGS technology as a diagnostic test with real-world examples of its utility in the fight against COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: High-Throughput Nucleotide Sequencing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Randomized controlled trials Limits: Humans Language: English Journal: Curr Issues Mol Biol Journal subject: Molecular Biology Year: 2021 Document Type: Article Affiliation country: Cimb43020061

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Full text: Available Collection: International databases Database: MEDLINE Main subject: High-Throughput Nucleotide Sequencing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study / Randomized controlled trials Limits: Humans Language: English Journal: Curr Issues Mol Biol Journal subject: Molecular Biology Year: 2021 Document Type: Article Affiliation country: Cimb43020061