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Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit.
Horova, Vladimira; Landova, Barbora; Hodek, Jan; Chalupsky, Karel; Krafcikova, Petra; Chalupska, Dominika; Duchoslav, Vojtech; Weber, Jan; Boura, Evzen; Klima, Martin.
  • Horova V; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Landova B; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Hodek J; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Chalupsky K; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Krafcikova P; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Chalupska D; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Duchoslav V; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Weber J; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Boura E; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
  • Klima M; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
Viruses ; 13(8)2021 07 29.
Article in English | MEDLINE | ID: covidwho-1335227
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2'-O MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2'-O RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA Caps / RNA, Viral / Viral Nonstructural Proteins / Viral Regulatory and Accessory Proteins / SARS-CoV-2 / COVID-19 / Methyltransferases Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: V13081487

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA Caps / RNA, Viral / Viral Nonstructural Proteins / Viral Regulatory and Accessory Proteins / SARS-CoV-2 / COVID-19 / Methyltransferases Limits: Humans Language: English Year: 2021 Document Type: Article Affiliation country: V13081487