Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit.
Viruses
; 13(8)2021 07 29.
Article
in English
| MEDLINE | ID: covidwho-1335227
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2'-O MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2'-O RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
RNA Caps
/
RNA, Viral
/
Viral Nonstructural Proteins
/
Viral Regulatory and Accessory Proteins
/
SARS-CoV-2
/
COVID-19
/
Methyltransferases
Limits:
Humans
Language:
English
Year:
2021
Document Type:
Article
Affiliation country:
V13081487
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