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Matrix biophysical cues direct mesenchymal stromal cell functions in immunity.
Wong, Sing Wan; Lenzini, Stephen; Giovanni, Regina; Knowles, Katherine; Shin, Jae-Won.
  • Wong SW; Department of Pharmacology and Regenerative Medicine, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA; Department of Biomedical Engineering, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.
  • Lenzini S; Department of Pharmacology and Regenerative Medicine, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA; Department of Biomedical Engineering, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.
  • Giovanni R; Department of Biomedical Science, University of Illinois College of Medicine, Rockford, IL, USA.
  • Knowles K; Department of Pharmacology and Regenerative Medicine, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA.
  • Shin JW; Department of Pharmacology and Regenerative Medicine, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA; Department of Biomedical Engineering, University of Illinois at Chicago, College of Medicine, Chicago, IL, USA. Electronic address: shinjw@uic.edu.
Acta Biomater ; 133: 126-138, 2021 10 01.
Article in English | MEDLINE | ID: covidwho-1340020
ABSTRACT
Hydrogels have been used to design synthetic matrices that capture salient features of matrix microenvironments to study and control cellular functions. Recent advances in understanding of both extracellular matrix biology and biomaterial design have shown that biophysical cues are powerful mediators of cell biology, especially that of mesenchymal stromal cells (MSCs). MSCs have been tested in many clinical trials because of their ability to modulate immune cells in different pathological conditions. While roles of biophysical cues in MSC biology have been studied in the context of multilineage differentiation, their significance in regulating immunomodulatory functions of MSCs is just beginning to be elucidated. This review first describes design principles behind how biophysical cues in native microenvironments influence the ability of MSCs to regulate immune cell production and functions. We will then discuss how biophysical cues can be leveraged to optimize cell isolation, priming, and delivery, which can help improve the success of MSC therapy for immunomodulation. Finally, a perspective is presented on how implementing biophysical cues in MSC potency assay can be important in predicting clinical outcomes. STATEMENT OF

SIGNIFICANCE:

Stromal cells of mesenchymal origin are known to direct immune cell functions in vivo by secreting paracrine mediators. This property has been leveraged in developing mesenchymal stromal cell (MSC)-based therapeutics by adoptive transfer to treat immunological rejection and tissue injuries, which have been tested in over one thousand clinical trials to date, but with mixed success. Advances in biomaterial design have enabled precise control of biophysical cues based on how stromal cells interact with the extracellular matrix in microenvironments in situ. Investigators have begun to use this approach to understand how different matrix biophysical parameters, such as fiber orientation, porosity, dimensionality, and viscoelasticity impact stromal cell-mediated immunomodulation. The insights gained from this effort can potentially be used to precisely define the microenvironmental cues for isolation, priming, and delivery of MSCs, which can be tailored based on different disease indications for optimal therapeutic outcomes.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Mesenchymal Stem Cells Type of study: Prognostic study Language: English Journal: Acta Biomater Year: 2021 Document Type: Article Affiliation country: J.actbio.2021.07.075

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Mesenchymal Stem Cells Type of study: Prognostic study Language: English Journal: Acta Biomater Year: 2021 Document Type: Article Affiliation country: J.actbio.2021.07.075