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A Sanger sequencing protocol for SARS-CoV-2 S-gene.
Daniels, Rodney S; Harvey, Ruth; Ermetal, Burcu; Xiang, Zheng; Galiano, Monica; Adams, Lorin; McCauley, John W.
  • Daniels RS; Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza), The Francis Crick Institute, London, UK.
  • Harvey R; Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza), The Francis Crick Institute, London, UK.
  • Ermetal B; Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza), The Francis Crick Institute, London, UK.
  • Xiang Z; Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza), The Francis Crick Institute, London, UK.
  • Galiano M; Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza), The Francis Crick Institute, London, UK.
  • Adams L; Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza), The Francis Crick Institute, London, UK.
  • McCauley JW; Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza), The Francis Crick Institute, London, UK.
Influenza Other Respir Viruses ; 15(6): 707-710, 2021 11.
Article in English | MEDLINE | ID: covidwho-1341261
ABSTRACT
We describe a Sanger sequencing protocol for SARS-CoV-2 S-gene the Spike (S)-glycoprotein product of which, composed of receptor-binding (S1) and membrane fusion (S2) segments, is the target of vaccines used to combat COVID-19. The protocol can be used in laboratories with basic Sanger sequencing capabilities and allows rapid "at source" screening for SARS-CoV-2 variants, notably those of concern. The protocol has been applied for surveillance, with clinical specimens collected in either nucleic acid preservation lysis-mix or virus transport medium, and research involving cultured viruses, and can yield data of public health importance in a timely manner.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Prognostic study Topics: Vaccines / Variants Limits: Humans Language: English Journal: Influenza Other Respir Viruses Journal subject: Virology Year: 2021 Document Type: Article Affiliation country: Irv.12892

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Prognostic study Topics: Vaccines / Variants Limits: Humans Language: English Journal: Influenza Other Respir Viruses Journal subject: Virology Year: 2021 Document Type: Article Affiliation country: Irv.12892