Orientation of immobilized antigens on common surfaces by a simple computational model: Exposition of SARS-CoV-2 Spike protein RBD epitopes.
Biophys Chem
; 265: 106441, 2020 10.
Article
in English
| MEDLINE | ID: covidwho-1343142
ABSTRACT
The possibility of immobilizing a protein with antigenic properties on a solid support offers significant possibilities in the development of immunosensors and vaccine formulations. For both applications, the orientation of the antigen should ensure ready accessibility of the antibodies to the epitope. However, an experimental assessment of the orientational preferences necessarily proceeds through the preparation/isolation of the antigen, the immobilization on different surfaces and one or more biophysical characterization steps. To predict a priori whether favorable orientations can be achieved or not would allow one to select the most promising experimental routes, partly mitigating the time cost towards the final product. In this manuscript, we apply a simple computational model, based on united-residue modelling, to the prediction of the orientation of the receptor binding domain of the SARS-CoV-2 spike protein on surfaces commonly used in lateral-flow devices. These calculations can account for the experimental observation that direct immobilization on gold gives sufficient exposure of the epitope to obtain a response in immunochemical assays.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Models, Molecular
/
Spike Glycoprotein, Coronavirus
/
Betacoronavirus
/
Epitopes
Type of study:
Observational study
/
Prognostic study
Topics:
Vaccines
Language:
English
Journal:
Biophys Chem
Year:
2020
Document Type:
Article
Affiliation country:
J.bpc.2020.106441
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