Preparation of Samples for Large-Scale Automated Electron Microscopy of Tissue and Cell Ultrastructure.
Microsc Microanal
; 27(4): 815-827, 2021 08.
Article
in English
| MEDLINE | ID: covidwho-1345523
ABSTRACT
Manual selection of targets in experimental or diagnostic samples by transmission electron microscopy (TEM), based on single overview and detail micrographs, has been time-consuming and susceptible to bias. Substantial information and throughput gain may now be achieved by the automated acquisition of virtually all structures in a given EM section. Resulting datasets allow the convenient pan-and-zoom examination of tissue ultrastructure with preserved microanatomical orientation. The technique is, however, critically sensitive to artifacts in sample preparation. We, therefore, established a methodology to prepare large-scale digitization samples (LDS) designed to acquire entire sections free of obscuring flaws. For evaluation, we highlight the supreme performance of scanning EM in transmission mode compared with other EM technology. The use of LDS will substantially facilitate access to EM data for a broad range of applications.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Specimen Handling
/
Microscopy, Electron, Scanning
Type of study:
Experimental Studies
Language:
English
Journal:
Microsc Microanal
Year:
2021
Document Type:
Article
Affiliation country:
S1431927621011958
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