A glycosaminoglycan microarray identifies the binding of SARS-CoV-2 spike protein to chondroitin sulfate E.
FEBS Lett
; 595(18): 2341-2349, 2021 09.
Article
in English
| MEDLINE | ID: covidwho-1347384
ABSTRACT
Heparan sulfate (HS), a sulfated glycosaminoglycan (GAG), was reported to be a necessary host attachment factor that promotes SARS-CoV-2 infection. In this study, we developed GAG microarrays based on fluorescence detection for high-sensitivity screening of the GAG-binding specificity of proteins and applied it for the analysis of SARS-CoV-2 spike (S) protein. Among the 20 distinct GAGs, the S protein bound not only to heparin (HEP)/HS but also to chondroitin sulfate E (CSE) in a concentration-dependent manner. We then analyzed the specificity of each subunit of the S protein. While the S1 subunit showed exclusive binding to HEP, the S2 subunit also bound to CSE and HEP/HS. CSE might act as an alternative attachment factor for HS in SARS-CoV-2 infection.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Chondroitin Sulfates
/
Spike Glycoprotein, Coronavirus
/
Glycosaminoglycans
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
FEBS Lett
Year:
2021
Document Type:
Article
Affiliation country:
1873-3468.14173
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