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The method utilized to purify the SARS-CoV-2 N protein can affect its molecular properties.
Tarczewska, Aneta; Kolonko-Adamska, Marta; Zarebski, Miroslaw; Dobrucki, Jurek; Ozyhar, Andrzej; Greb-Markiewicz, Beata.
  • Tarczewska A; Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland. Electronic address: aneta.tarczewska@pwr.edu.pl.
  • Kolonko-Adamska M; Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland. Electronic address: marta.kolonko@pwr.edu.pl.
  • Zarebski M; Department of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland.
  • Dobrucki J; Department of Cell Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland.
  • Ozyhar A; Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
  • Greb-Markiewicz B; Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland. Electronic address: beata.greb-markiewicz@pwr.edu.pl.
Int J Biol Macromol ; 188: 391-403, 2021 Oct 01.
Article in English | MEDLINE | ID: covidwho-1347646
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ABSTRACT
One of the main structural proteins of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the nucleocapsid protein (N). The basic function of this protein is to bind genomic RNA and to form a protective nucleocapsid in the mature virion. The intrinsic ability of the N protein to interact with nucleic acids makes its purification very challenging. Therefore, typically employed purification methods appear to be insufficient for removing nucleic acid contamination. In this study, we present a novel purification protocol that enables the N protein to be prepared without any bound nucleic acids. We also performed comparative structural analysis of the N protein contaminated with nucleic acids and free of contamination and showed significant differences in the structural and phase separation properties of the protein. These results indicate that nucleic-acid contamination may severely affect molecular properties of the purified N protein. In addition, the notable ability of the N protein to form condensates whose morphology and behaviour suggest more ordered forms resembling gel-like or solid structures is described.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Liquid-Liquid Extraction / Coronavirus Nucleocapsid Proteins / SARS-CoV-2 Language: English Journal: Int J Biol Macromol Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Liquid-Liquid Extraction / Coronavirus Nucleocapsid Proteins / SARS-CoV-2 Language: English Journal: Int J Biol Macromol Year: 2021 Document Type: Article