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Pulsed blue light inactivates two strains of human coronavirus.
Enwemeka, Chukuka S; Bumah, Violet V; Mokili, John L.
  • Enwemeka CS; College of Health and Human Services, San Diego State University, San Diego, CA, USA. Electronic address: Enwemeka@sdsu.edu.
  • Bumah VV; College of Health and Human Services, San Diego State University, San Diego, CA, USA.
  • Mokili JL; Viral Information Institute, Department of Biology, College of Sciences, San Diego State University, San Diego, CA, USA.
J Photochem Photobiol B ; 222: 112282, 2021 Sep.
Article in English | MEDLINE | ID: covidwho-1347724
ABSTRACT
Emerging evidence suggests that blue light has the potential to inactivate viruses. Therefore, we investigated the effect of 405 nm, 410 nm, 425 nm and 450 nm pulsed blue light (PBL) on human alpha coronavirus HCoV-229 E and human beta coronavirus HCoV-OC43, using Qubit fluorometry and RT-LAMP to quantitate the amount of nucleic acid in irradiated and control samples. Like SARS-CoV-2, HCoV-229E and HCoV-OC43 are single stranded RNA viruses transmitted by air and direct contact; they have similar genomic sizes as SARS-CoV-2, and are used as surrogates for SARS-CoV-2. Irradiation was carried out either at 32.4 J cm-2 using 3 mW cm-2 irradiance or at 130 J cm-2 using 12 mW cm-2 irradiance.

Results:

(1) At each wavelength tested, PBL was antiviral against both coronaviruses. (2) 405 nm light gave the best result, yielding 52.3% (2.37 log10) inactivation against HCoV-OC43 (p < .0001), and a significant 1.46 log 10 (44%) inactivation of HCoV-229E (p < .01). HCoV-OC43, which like SARS-CoV-2 is a beta coronavirus, was more susceptible to PBL irradiation than alpha coronavirus HCoV-229E. The latter finding suggests that PBL is potentially antiviral against multiple coronavirus strains, and that, while its potency may vary from one virus to another, it seems more antiviral against beta coronaviruses, such as HCoV-OC43. (3) Further, the antiviral effect of PBL was better at a higher irradiance than a lower irradiance, and this indicates that with further refinement, a protocol capable of yielding 100% inactivation of viruses is attainable.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus 229E, Human / Coronavirus OC43, Human / Low-Level Light Therapy / SARS-CoV-2 Limits: Humans Language: English Journal: J Photochem Photobiol B Journal subject: Biology Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Coronavirus 229E, Human / Coronavirus OC43, Human / Low-Level Light Therapy / SARS-CoV-2 Limits: Humans Language: English Journal: J Photochem Photobiol B Journal subject: Biology Year: 2021 Document Type: Article