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Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells.
Schubert, Maren; Nimtz, Manfred; Bertoglio, Federico; Schmelz, Stefan; Lukat, Peer; van den Heuvel, Joop.
  • Schubert M; Department of Biotechnology, Institut fuer Biochemie, Biotechnologie und Bioinformatik, Technische Universitaet Braunschweig, Braunschweig, Germany.
  • Nimtz M; RG Cellular Proteomics, Department of Structure and Function of Proteins, Helmholtz Zentrum für Infektionsforschung GmbH, Braunschweig, Germany.
  • Bertoglio F; Department of Biotechnology, Institut fuer Biochemie, Biotechnologie und Bioinformatik, Technische Universitaet Braunschweig, Braunschweig, Germany.
  • Schmelz S; Department of Structure and Function of Proteins, Helmholtz Zentrum für Infektionsforschung GmbH, Braunschweig, Germany.
  • Lukat P; Department of Structure and Function of Proteins, Helmholtz Zentrum für Infektionsforschung GmbH, Braunschweig, Germany.
  • van den Heuvel J; Department of Structure and Function of Proteins, Helmholtz Zentrum für Infektionsforschung GmbH, Braunschweig, Germany. jvh@helmholtz-hzi.de.
Methods Mol Biol ; 2305: 129-140, 2021.
Article in English | MEDLINE | ID: covidwho-1355903
ABSTRACT
The expression of mammalian recombinant proteins in insect cell lines using transient-plasmid-based gene expression enables the production of high-quality protein samples. Here, the procedure for virus-free transient gene expression (TGE) in High Five insect cells is described in detail. The parameters that determine the efficiency and reproducibility of the method are presented in a robust protocol for easy implementation and set-up of the method. The applicability of the TGE method in High Five cells for proteomic, structural, and functional analysis of the expressed proteins is shown.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biotechnology / Transfection / Cloning, Molecular / Spike Glycoprotein, Coronavirus / Insecta Limits: Animals / Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2021 Document Type: Article Affiliation country: 978-1-0716-1406-8_6

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biotechnology / Transfection / Cloning, Molecular / Spike Glycoprotein, Coronavirus / Insecta Limits: Animals / Humans Language: English Journal: Methods Mol Biol Journal subject: Molecular Biology Year: 2021 Document Type: Article Affiliation country: 978-1-0716-1406-8_6