Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells.
Methods Mol Biol
; 2305: 129-140, 2021.
Article
in English
| MEDLINE | ID: covidwho-1355903
ABSTRACT
The expression of mammalian recombinant proteins in insect cell lines using transient-plasmid-based gene expression enables the production of high-quality protein samples. Here, the procedure for virus-free transient gene expression (TGE) in High Five insect cells is described in detail. The parameters that determine the efficiency and reproducibility of the method are presented in a robust protocol for easy implementation and set-up of the method. The applicability of the TGE method in High Five cells for proteomic, structural, and functional analysis of the expressed proteins is shown.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Biotechnology
/
Transfection
/
Cloning, Molecular
/
Spike Glycoprotein, Coronavirus
/
Insecta
Limits:
Animals
/
Humans
Language:
English
Journal:
Methods Mol Biol
Journal subject:
Molecular Biology
Year:
2021
Document Type:
Article
Affiliation country:
978-1-0716-1406-8_6
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