Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins.
STAR Protoc
; 2(4): 100781, 2021 12 17.
Article
in English
| MEDLINE | ID: covidwho-1356489
ABSTRACT
We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR to measure endogenous mRNA induction. The assay requires stringent normalization and confirmation of the results in more physiological settings. The protocol is adaptable for other viruses and other innate immune stimuli. For complete details on the use and execution of this protocol, please refer to Hayn et al. (2021).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Viral Proteins
/
RNA, Messenger
/
Gene Expression Regulation, Viral
/
Interferons
/
SARS-CoV-2
/
COVID-19
/
Luciferases
Limits:
Humans
Language:
English
Journal:
STAR Protoc
Year:
2021
Document Type:
Article
Affiliation country:
J.xpro.2021.100781
Similar
MEDLINE
...
LILACS
LIS