Efficacy of Silene arenosa extract on acetylcholinesterase in Bungarus sindanus (krait) venom.
J Tradit Chin Med
; 41(3): 349-354, 2021 06.
Article
in English
| MEDLINE | ID: covidwho-1357700
ABSTRACT
OBJECTIVE:
To examine the efficacy of Silene arenosa extract on acetylcholinesterase (AChE) of krait (Bungarus Sindanus) snake venom.METHODS:
The present project designed to evaluate the inhibition of AChE by following standard procedures.RESULTS:
Statistical analysis of the results showed that Silene arenosa exerted 73% inhibition against the krait venom acetylcholinesterase at fixed substrate acetylcholine (ACh) concentration (0.5 mM). Kinetic analysis using the Lineweaver Burk plot revealed that Silene arenosa caused a competitive type of inhibition i.e. Km values increased from 26.6 to 93.3 mM (26.6% to 93.3%) and Vmax remained constant in a concentration-dependent manner. Silene arenosa competes with the substrate to bind at the active site of the enzyme. The Kmapp of venom AChE for Silene arenosa increased from 60% to 81.6% and the Vmaxapp remains constant. Ki (inhibition constant was estimated to be 48 µg for snake venom; while the Km (Michaelis-Menten constant of AChE- substrate into AChE and product) was estimated to be 0.5 mM. The IC50 of AchE calculated for Silene arenosa was 67 µg.CONCLUSION:
The present results suggest that Silene arenosa extract can be considered as an inhibitor of snake venom AChE.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Acetylcholinesterase
/
Silene
Type of study:
Experimental Studies
Limits:
Animals
/
Humans
Language:
English
Journal:
J Tradit Chin Med
Year:
2021
Document Type:
Article
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