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Rapid COVID-19 antigenic tests: Usefulness of a modified method for diagnosis.
Soleimani, Reza; Deckers, Corentin; Huang, Te-Din; Bogaerts, Pierre; Evrard, Stéphanie; Wallemme, Isaline; Habib, Boutaina; Rouzé, Pauline; Denis, Olivier.
  • Soleimani R; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
  • Deckers C; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
  • Huang TD; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
  • Bogaerts P; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
  • Evrard S; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
  • Wallemme I; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
  • Habib B; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
  • Rouzé P; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
  • Denis O; Department of Laboratory Medicine, Service of Medical Microbiology, CHU UCL Namur, Université Catholique de Louvain, Yvoir, Belgium.
J Med Virol ; 93(9): 5655-5659, 2021 Sep.
Article in English | MEDLINE | ID: covidwho-1363696
ABSTRACT
The current reliable recommended test for coronavirus disease 2019 (COVID-19) diagnosis is quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Rapid antigen test devices could be useful as they are less expensive, faster without the need of specialized laboratories to perform the test. We report the performances of two rapid immunochromatographic antigen testing devices compared with RT-qPCR for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection in nasopharyngeal samples. We carried out a lateral-flow tests study on 401 nasopharyngeal swab samples from nonduplicated suspected COVID-19 subjects. An equal volume of universal transport medium tubes-containing samples (dilution ratio = 115) were added to the manufacturer's extraction buffer solution (dilution ratio = 12) and analyzed on BioSpeedia COVID19Speed-Antigen Test and on Abbott Panbio™ COVID-19 Ag Rapid Test, devices. Qualitative results were compared to those obtained by the RT-qPCR (Allplex™ SARS-CoV-2 Assay Seegene). Based on our data, the overall sensitivity for BioSpeedia and Panbio devices was estimated at 65.5% and 75.0%, respectively. The sensitivity was greater for cycle threshold values less than 25 achieving 90.4 and 96.8 for BioSpeedia and Panbio devices, respectively. A perfect specificity of 100.0% was observed for both devices.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Qualitative research Limits: Humans Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.27094

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Qualitative research Limits: Humans Language: English Journal: J Med Virol Year: 2021 Document Type: Article Affiliation country: Jmv.27094