A microfluidic chip for visual investigation of the interaction of nanoemulsion of Satureja Khuzistanica essential oil and a model gram-negative bacteria.
Int J Pharm
; 607: 121032, 2021 Sep 25.
Article
in English
| MEDLINE | ID: covidwho-1364122
ABSTRACT
Nanotechnology has provided novel approaches against food born and pathogenic bacteria. Within the present study, the effects of pure and nanoemulsified essential oil derived from Satureja Khuzistanica essential oil (SKEO) on Escherichia coli (E. coli ATCC 25922) as a human pathogen has been studied using a microfluidic chip. The morphology and antibacterial activity of E. coli at disparate residence durations (from 2 to 30 min) and various nanoemulsified or pure essential oil concentrations (8.0-62.5 µg mL-1) and numerous nanoemulsion's droplet sizes from 32 to 124 nm, have been investigated in the microfluidic system. Also, the quantitative analysis including optical density, time killing assay, protein, nucleic acid and potassium release were employed to confirm the effects of bacterial inhibition taking advantage of the chip apparatus. It was revealed that the prepared nanoemulsion left a considerable destructive effect on E. coli bacterial membrane, confirmed by fast release of cytoplasmic elements including protein, nucleic acid and potassium. However, this process was remarkably intensified for both nanoemulsion and pure essential oil using the microfluidic chip versus the conventional methods. The results also revealed that after 4 min of bacterium treatment by 12.5 µg mL-1 nanoemulsion with 32 nm mean particle size, the bacterial membrane wall began to degrade rapidly, and bacterial activity was almost completely inhibited in a 20-min period. These findings may have implications in the similarly structured and phospholipid-encapsulated bacteria and viruses, like COVID-19.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Oils, Volatile
/
Satureja
/
COVID-19
Limits:
Humans
Language:
English
Journal:
Int J Pharm
Year:
2021
Document Type:
Article
Affiliation country:
J.ijpharm.2021.121032
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