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Rapid Base-Specific Calling of SARS-CoV-2 Variants of Concern Using Combined RT-PCR Melting Curve Screening and SIRPH Technology.
Tierling, Sascha; Kattler, Kathrin; Vogelgesang, Markus; Pfuhl, Thorsten; Lohse, Stefan; Lo Porto, Christina; Schmitt, Beate; Nastasja, Seiwert; Salhab, Abdulrahman; Smola, Sigrun; Walter, Jörn.
  • Tierling S; Department of Genetics, Saarland University, Saarbrücken, Saarland, Germany.
  • Kattler K; Department of Genetics, Saarland University, Saarbrücken, Saarland, Germany.
  • Vogelgesang M; Institute of Virology, Saarland University Medical Center, Homburg, Saarland, Germany.
  • Pfuhl T; Institute of Virology, Saarland University Medical Center, Homburg, Saarland, Germany.
  • Lohse S; Institute of Virology, Saarland University Medical Center, Homburg, Saarland, Germany.
  • Lo Porto C; Department of Genetics, Saarland University, Saarbrücken, Saarland, Germany.
  • Schmitt B; Department of Genetics, Saarland University, Saarbrücken, Saarland, Germany.
  • Nastasja S; Institute of Virology, Saarland University Medical Center, Homburg, Saarland, Germany.
  • Salhab A; Department of Genetics, Saarland University, Saarbrücken, Saarland, Germany.
  • Smola S; Institute of Virology, Saarland University Medical Center, Homburg, Saarland, Germany.
  • Walter J; Department of Genetics, Saarland University, Saarbrücken, Saarland, Germany.
Open Forum Infect Dis ; 8(8): ofab364, 2021 Aug.
Article in English | MEDLINE | ID: covidwho-1364826
ABSTRACT

BACKGROUND:

The emergence of novel variants of concern of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands fast and reliable detection of such variants in local populations.

METHODS:

Here we present a cost-efficient and fast workflow combining a prescreening of SARS-CoV-2-positive samples using reverse transcription polymerase chain reaction melting curve analysis with multiplexed IP-RP-HPLC-based single nucleotide primer extensions.

RESULTS:

The entire workflow from positive SARS-CoV-2 testing to base-specific identification of variants requires about 24 hours.

CONCLUSIONS:

We applied the sensitive method to monitor local variant of concern outbreaks in SARS-CoV-2-positive samples collected in a confined region of Germany.
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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Topics: Variants Language: English Journal: Open Forum Infect Dis Year: 2021 Document Type: Article Affiliation country: Ofid

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Full text: Available Collection: International databases Database: MEDLINE Type of study: Diagnostic study Topics: Variants Language: English Journal: Open Forum Infect Dis Year: 2021 Document Type: Article Affiliation country: Ofid