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Characterization of the Human Oropharyngeal Microbiomes in SARS-CoV-2 Infection and Recovery Patients.
Gao, Ming; Wang, Haiyu; Luo, Hong; Sun, Ying; Wang, Ling; Ding, Suying; Ren, Hongyan; Gang, Jiaqi; Rao, Benchen; Liu, Shanshuo; Wang, Xuemei; Gao, Xinxin; Li, Mengyi; Zou, Yawen; Liu, Chao; Yuan, Chengyu; Sun, Jiarui; Cui, Guangying; Ren, Zhigang.
  • Gao M; Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Wang H; Gene Hospital of Henan Province; Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Luo H; Gene Hospital of Henan Province; Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Sun Y; Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Wang L; Guangshan County People's Hospital, Guangshan County, Xinyang, 465450, China.
  • Ding S; Gene Hospital of Henan Province; Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Ren H; Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Gang J; Department of Clinical Laboratory, Henan Provincial Chest Hospital, Zhengzhou, 450008, China.
  • Rao B; Health Management Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Liu S; Shanghai Mobio Biomedical Technology Co., Ltd., Shanghai, 201111, China.
  • Wang X; Xiuwu County People's Hospital, Xiuwu County, Jiaozuo, 454350, China.
  • Gao X; Gene Hospital of Henan Province; Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Li M; Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Zou Y; Gene Hospital of Henan Province; Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Liu C; Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Yuan C; Gene Hospital of Henan Province; Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Sun J; Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Cui G; Health Management Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
  • Ren Z; Department of Oncology, Zhengzhou First People's Hospital, Zhengzhou, 450004, China.
Adv Sci (Weinh) ; 8(20): e2102785, 2021 10.
Article in English | MEDLINE | ID: covidwho-1366208
ABSTRACT
Respiratory tract microbiome is closely related to respiratory tract infections, while characterization of oropharyngeal microbiome in recovered coronavirus disease 2019 (COVID-19) patients is not studied. Herein, oropharyngeal swabs are collected from confirmed cases (CCs) with COVID-19 (73 subjects), suspected cases (SCs) (36), confirmed cases who recovered (21), suspected cases who recovered (36), and healthy controls (Hs) (140) and then completed MiSeq sequencing. Oropharyngeal microbial α-diversity is markedly reduced in CCs versus Hs. Opportunistic pathogens are increased, while butyrate-producing genera are decreased in CCs versus Hs. The classifier based on eight optimal microbial markers is constructed through a random forest model and reached great diagnostic efficacy in both discovery and validation cohorts. Notably, the classifier successfully diagnosed SCs with positive IgG antibody as CCs and is demonstrated from the perspective of the microbiome. Importantly, several genera with significant differences gradually increase and decrease along with recovery from COVID-19. Forty-four oropharyngeal operational taxonomy units (OTUs) are closely correlated with 11 clinical indicators of SARS-CoV-2 infection and Hs based on Spearman correlation analysis. Together, this research is the first to characterize oropharyngeal microbiota in recovered COVID-19 cases and suspected cases, to successfully construct and validate the diagnostic model for COVID-19 and to depict the correlations between microbial OTUs and clinical indicators.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Oropharynx / Microbiota / SARS-CoV-2 / COVID-19 Type of study: Cohort study / Observational study / Prognostic study / Randomized controlled trials Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: Adv Sci (Weinh) Year: 2021 Document Type: Article Affiliation country: Advs.202102785

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Oropharynx / Microbiota / SARS-CoV-2 / COVID-19 Type of study: Cohort study / Observational study / Prognostic study / Randomized controlled trials Limits: Adult / Female / Humans / Male / Middle aged Language: English Journal: Adv Sci (Weinh) Year: 2021 Document Type: Article Affiliation country: Advs.202102785