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Assessment of humoral and cellular immunity induced by the BNT162b2 SARS-CoV-2 vaccine in healthcare workers, elderly people, and immunosuppressed patients with autoimmune disease.
Malipiero, Giacomo; Moratto, Anna; Infantino, Maria; D'Agaro, Pierlanfranco; Piscianz, Elisa; Manfredi, Mariangela; Grossi, Valentina; Benvenuti, Enrico; Bulgaresi, Matteo; Benucci, Maurizio; Villalta, Danilo.
  • Malipiero G; Immunology and Allergy Unit, Ospedale S-Maria degli Angeli, Pordenone, Italy.
  • Moratto A; Immunology and Allergy Unit, Ospedale S-Maria degli Angeli, Pordenone, Italy.
  • Infantino M; Immunology and Allergy Laboratory Unit, San Giovanni Di Dio Hospital, Florence, Italy.
  • D'Agaro P; Laboratory for Hygiene and Public Health, University Hospital of Trieste, Trieste, Italy.
  • Piscianz E; Laboratory for Hygiene and Public Health, University Hospital of Trieste, Trieste, Italy.
  • Manfredi M; Immunology and Allergy Laboratory Unit, San Giovanni Di Dio Hospital, Florence, Italy.
  • Grossi V; Immunology and Allergy Laboratory Unit, San Giovanni Di Dio Hospital, Florence, Italy.
  • Benvenuti E; Geriatric Unit Firenze-Empoli, Santa Maria Annunziata Hospital, Florence, Italy.
  • Bulgaresi M; Geriatric Unit Firenze-Empoli, Santa Maria Annunziata Hospital, Florence, Italy.
  • Benucci M; Rheumatology Unit, San Giovanni Di Dio Hospital, Florence, Italy.
  • Villalta D; Immunology and Allergy Unit, Ospedale S-Maria degli Angeli, Pordenone, Italy. danilo.villalta@asfo.sanita.fvg.it.
Immunol Res ; 69(6): 576-583, 2021 12.
Article in English | MEDLINE | ID: covidwho-1366407
ABSTRACT
The development of vaccines to prevent SARS-CoV-2 infection has mainly relied on the induction of neutralizing antibodies (nAbs) to the Spike protein of SARS-CoV-2, but there is growing evidence that T cell immune response can contribute to protection as well. In this study, an anti-receptor binding domain (RBD) antibody assay and an INFγ-release assay (IGRA) were used to detect humoral and cellular responses to the Pfizer-BioNTech BNT162b2 vaccine in three separate cohorts of COVID-19-naïve patients 108 healthcare workers (HCWs), 15 elderly people, and 5 autoimmune patients treated with immunosuppressive agents. After the second dose of vaccine, the mean values of anti-RBD antibodies (Abs) and INFγ were 123.33 U/mL (range 27.55-464) and 1513 mIU/mL (range 145-2500) in HCWs and 210.7 U/mL (range 3-500) and 1167 mIU/mL (range 83-2500) in elderly people. No correlations between age and immune status were observed. On the contrary, a weak but significant positive correlation was found between INFγ and anti-RBD Abs values (rho = 0.354, p = 0.003). As to the autoimmune cohort, anti-RBD Abs were not detected in the two patients with absent peripheral CD19+B cells, despite high INFγ levels being observed in all 5 patients after vaccination. Even though the clinical relevance of T cell response has not yet been established as a correlate of vaccine-induced protection, IGRA testing has showed optimal sensitivity and specificity to define vaccine responders, even in patients lacking a cognate antibody response to the vaccine.
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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Immunocompromised Host / Immunity, Humoral / COVID-19 Vaccines / SARS-CoV-2 / Immunity, Cellular Subject: Immunocompromised Host / Immunity, Humoral / COVID-19 Vaccines / SARS-CoV-2 / Immunity, Cellular Language: English Journal: Immunol Res Clinical aspect: Etiology / Therapy Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Immunocompromised Host / Immunity, Humoral / COVID-19 Vaccines / SARS-CoV-2 / Immunity, Cellular Subject: Immunocompromised Host / Immunity, Humoral / COVID-19 Vaccines / SARS-CoV-2 / Immunity, Cellular Language: English Journal: Immunol Res Clinical aspect: Etiology / Therapy Year: 2021
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