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Ultrasensitive and Simultaneous Detection of Two Specific SARS-CoV-2 Antigens in Human Specimens Using Direct/Enrichment Dual-Mode Fluorescence Lateral Flow Immunoassay.
Wang, Chongwen; Cheng, Xiaodan; Liu, Liyan; Zhang, Xiaochang; Yang, Xingsheng; Zheng, Shuai; Rong, Zhen; Wang, Shengqi.
  • Wang C; College of Life Sciences, Anhui Agricultural University, Hefei 230036, P. R. China.
  • Cheng X; Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China.
  • Liu L; Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing 100850, P. R. China.
  • Zhang X; College of Life Sciences, Anhui Agricultural University, Hefei 230036, P. R. China.
  • Yang X; Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China.
  • Zheng S; Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China.
  • Rong Z; Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China.
  • Wang S; College of Life Sciences, Anhui Agricultural University, Hefei 230036, P. R. China.
ACS Appl Mater Interfaces ; 13(34): 40342-40353, 2021 Sep 01.
Article in English | MEDLINE | ID: covidwho-1366784
ABSTRACT
Sensitive point-of-care methods for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in clinical specimens are urgently needed to achieve rapid screening of viral infection. We developed a magnetic quantum dot-based dual-mode lateral flow immunoassay (LFIA) biosensor for the high-sensitivity simultaneous detection of SARS-CoV-2 spike (S) and nucleocapsid protein (NP) antigens, which is beneficial for improving the detection accuracy and efficiency of SARS-CoV-2 infection in the point-of-care testing area. A high-performance magnetic quantum dot with a triple-QD shell (MagTQD) nanotag was first fabricated and integrated into the LFIA system to provide superior fluorescence signals, enrichment ability, and detectability for S/NP antigen testing. Two detection modes were provided by the proposed MagTQD-LFIA. The direct mode was used for rapid screening or urgent detection of suspected samples within 10 min, and the enrichment mode was used for the highly sensitive and quantitative analysis of SARS-CoV-2 antigens in biological samples without the interference of the "hook effect." The simultaneous detection of SARS-CoV-2 S/NP antigens was conducted in one LFIA strip, and the detection limits for two antigens under direct and enrichment modes were 1 and 0.5 pg/mL, respectively. The MagTQD-LFIA showed high accuracy, specificity, and stability in saliva and nasal swab samples and is an efficient tool with flexibility to meet the testing requirements for SARS-CoV-2 antigens in various situations.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Spike Glycoprotein, Coronavirus / Coronavirus Nucleocapsid Proteins / SARS-CoV-2 / Antigens, Viral Type of study: Diagnostic study Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Spike Glycoprotein, Coronavirus / Coronavirus Nucleocapsid Proteins / SARS-CoV-2 / Antigens, Viral Type of study: Diagnostic study Limits: Humans Language: English Journal: ACS Appl Mater Interfaces Journal subject: Biotechnology / Biomedical Engineering Year: 2021 Document Type: Article