Human small intestinal infection by SARS-CoV-2 is characterized by a mucosal infiltration with activated CD8+ T cells.
Mucosal Immunol
; 14(6): 1381-1392, 2021 11.
Article
in English
| MEDLINE | ID: covidwho-1366810
ABSTRACT
The SARS-CoV-2 pandemic has so far claimed over three and a half million lives worldwide. Though the SARS-CoV-2 mediated disease COVID-19 has first been characterized by an infection of the upper airways and the lung, recent evidence suggests a complex disease including gastrointestinal symptoms. Even if a direct viral tropism of intestinal cells has recently been demonstrated, it remains unclear, whether gastrointestinal symptoms are caused by direct infection of the gastrointestinal tract by SARS-CoV-2 or whether they are a consequence of a systemic immune activation and subsequent modulation of the mucosal immune system. To better understand the cause of intestinal symptoms we analyzed biopsies of the small intestine from SARS-CoV-2 infected individuals. Applying qRT-PCR and immunohistochemistry, we detected SARS-CoV-2 RNA and nucleocapsid protein in duodenal mucosa. In addition, applying imaging mass cytometry and immunohistochemistry, we identified histomorphological changes of the epithelium, which were characterized by an accumulation of activated intraepithelial CD8+ T cells as well as epithelial apoptosis and subsequent regenerative proliferation in the small intestine of COVID-19 patients. In summary, our findings indicate that intraepithelial CD8+ T cells are activated upon infection of intestinal epithelial cells with SARS-CoV-2, providing one possible explanation for gastrointestinal symptoms associated with COVID-19.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Lymphocyte Activation
/
CD8-Positive T-Lymphocytes
/
Immunity, Mucosal
/
Duodenum
/
Intraepithelial Lymphocytes
/
SARS-CoV-2
/
COVID-19
/
Intestinal Diseases
/
Intestinal Mucosa
Type of study:
Observational study
Limits:
Adult
/
Aged
/
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mucosal Immunol
Journal subject:
Allergy and Immunology
Year:
2021
Document Type:
Article
Affiliation country:
S41385-021-00437-z
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