Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products.
Biotechniques
; 70(2): 126-133, 2021 02.
Article
in English
| MEDLINE | ID: covidwho-1369584
ABSTRACT
Biopharmaceutical products are of great importance in the treatment or prevention of many diseases and represent a growing share of the global pharmaceutical market. The usual technology for protein synthesis (cell-based expression) faces certain obstacles, especially with 'difficult-to-express' proteins. Cell-free protein synthesis (CFPS) can overcome the main bottlenecks of cell-based expression. This review aims to present recent advances in the production process of biologic products by CFPS. First, key aspects of CFPS systems are summarized. A description of several biologic products that have been successfully produced using the CFPS system is provided. Finally, the CFPS system's ability to scale up and scale down, its main limitations and its application for biologics production are discussed.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Protein Biosynthesis
/
Biological Products
/
Cell-Free System
Language:
English
Journal:
Biotechniques
Year:
2021
Document Type:
Article
Affiliation country:
BTN-2020-0155
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