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Optical trapping assisted label-free and amplification-free detection of SARS-CoV-2 RNAs with an optofluidic nanopore sensor.
Sampad, Mohammad Julker Neyen; Zhang, Han; Yuzvinsky, Thomas D; Stott, Matthew A; Hawkins, Aaron R; Schmidt, Holger.
  • Sampad MJN; School of Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA.
  • Zhang H; School of Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA.
  • Yuzvinsky TD; School of Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA.
  • Stott MA; ECEn Department, Brigham Young University, 450 Engineering Building, Provo, UT, 84602, USA.
  • Hawkins AR; ECEn Department, Brigham Young University, 450 Engineering Building, Provo, UT, 84602, USA.
  • Schmidt H; School of Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA. Electronic address: hschmidt@soe.ucsc.edu.
Biosens Bioelectron ; 194: 113588, 2021 Dec 15.
Article in English | MEDLINE | ID: covidwho-1372896
ABSTRACT
Ultrasensitive, versatile sensors for molecular biomarkers are a critical component of disease diagnostics and personalized medicine as the COVID-19 pandemic has revealed in dramatic fashion. Integrated electrical nanopore sensors can fill this need via label-free, direct detection of individual biomolecules, but a fully functional device for clinical sample analysis has yet to be developed. Here, we report amplification-free detection of SARS-CoV-2 RNAs with single molecule sensitivity from clinical nasopharyngeal swab samples on an electro-optofluidic chip. The device relies on optically assisted delivery of target carrying microbeads to the nanopore for single RNA detection after release. A sensing rate enhancement of over 2,000x with favorable scaling towards lower concentrations is demonstrated. The combination of target specificity, chip-scale integration and rapid detection ensures the practicality of this approach for COVID-19 diagnosis over the entire clinically relevant concentration range from 104-109 copies/mL.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Nanopores / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2021 Document Type: Article Affiliation country: J.bios.2021.113588

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Nanopores / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: Biosens Bioelectron Journal subject: Biotechnology Year: 2021 Document Type: Article Affiliation country: J.bios.2021.113588