An antigen microarray protocol for COVID-19 serological analysis.
STAR Protoc
; 2(3): 100815, 2021 09 17.
Article
in English
| MEDLINE | ID: covidwho-1373301
ABSTRACT
The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Microarray Analysis
/
Spike Glycoprotein, Coronavirus
/
SARS-CoV-2
/
COVID-19
/
Antibodies, Viral
/
Antigens, Viral
Limits:
Humans
Language:
English
Journal:
STAR Protoc
Year:
2021
Document Type:
Article
Affiliation country:
J.xpro.2021.100815
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