Application of pseudotyped virus particles to monitor Ebola virus and SARS-CoV-2 viral entry in human cell lines.
STAR Protoc
; 2(4): 100818, 2021 12 17.
Article
in English
| MEDLINE | ID: covidwho-1373302
ABSTRACT
Experimental work on highly pathogenic viruses such as Ebola virus (EBOV) and severe acute respiratory syndrome coronavirus-2 requires high-level biosafety facilities. Here, we provide a detailed step-by-step protocol which details the production and application of replication-incompetent murine leukemia virus-based pseudotyped particles to monitor and quantify the viral entry efficiency in human cell lines under biosafety level-2 conditions. We describe the use of viral particles encoding luciferase gene and the quantification of transduction efficiency by measuring luciferase activity. For complete details on the use and execution of this protocol, please refer to Imre et al. (2021).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Hemorrhagic Fever, Ebola
/
Ebolavirus
/
Virus Internalization
/
SARS-CoV-2
/
COVID-19
/
Viral Pseudotyping
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
STAR Protoc
Year:
2021
Document Type:
Article
Affiliation country:
J.xpro.2021.100818
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