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Comparative transcriptomic analysis of SARS-CoV-2 infected cell model systems reveals differential innate immune responses.
Sun, Guihua; Cui, Qi; Garcia, Gustavo; Wang, Cheng; Zhang, Mingzi; Arumugaswami, Vaithilingaraja; Riggs, Arthur D; Shi, Yanhong.
  • Sun G; Department of Diabetes Complications & Metabolism, Beckman Research Institute of City of Hope, Duarte, CA, 91010, USA.
  • Cui Q; Division of Stem Cell Biology Research, Department of Developmental and Stem Cell Biology, Beckman Research Institute of City of Hope, Duarte, CA, 91010, USA.
  • Garcia G; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
  • Wang C; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, 90095, USA.
  • Zhang M; Division of Stem Cell Biology Research, Department of Developmental and Stem Cell Biology, Beckman Research Institute of City of Hope, Duarte, CA, 91010, USA.
  • Arumugaswami V; Division of Stem Cell Biology Research, Department of Developmental and Stem Cell Biology, Beckman Research Institute of City of Hope, Duarte, CA, 91010, USA.
  • Riggs AD; Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA. VArumugaswami@mednet.ucla.edu.
  • Shi Y; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, 90095, USA. VArumugaswami@mednet.ucla.edu.
Sci Rep ; 11(1): 17146, 2021 08 25.
Article in English | MEDLINE | ID: covidwho-1373450
ABSTRACT
The transcriptome of SARS-CoV-2-infected cells that reflects the interplay between host and virus has provided valuable insights into mechanisms underlying SARS-CoV-2 infection and COVID-19 disease progression. In this study, we show that SARS-CoV-2 can establish a robust infection in HEK293T cells that overexpress human angiotensin-converting enzyme 2 (hACE2) without triggering significant host immune response. Instead, endoplasmic reticulum stress and unfolded protein response-related pathways are predominantly activated. By comparing our data with published transcriptome of SARS-CoV-2 infection in other cell lines, we found that the expression level of hACE2 directly correlates with the viral load in infected cells but not with the scale of immune responses. Only cells that express high level of endogenous hACE2 exhibit an extensive immune attack even with a low viral load. Therefore, the infection route may be critical for the extent of the immune response, thus the severity of COVID-19 disease status.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Gene Expression Profiling / SARS-CoV-2 / Immunity, Innate Type of study: Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-96462-w

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Gene Expression Profiling / SARS-CoV-2 / Immunity, Innate Type of study: Prognostic study Limits: Humans Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-96462-w