Your browser doesn't support javascript.
Magnetization Transfer to Enhance NOE Cross-Peaks among Labile Protons: Applications to Imino-Imino Sequential Walks in SARS-CoV-2-Derived RNAs.
Novakovic, Mihajlo; Kupce, Eriks; Scherf, Tali; Oxenfarth, Andreas; Schnieders, Robbin; Grün, J Tassilo; Wirmer-Bartoschek, Julia; Richter, Christian; Schwalbe, Harald; Frydman, Lucio.
  • Novakovic M; Department of Chemical and Biological Physics Weizmann Institute of Science 7610001 Rehovot Israel.
  • Kupce E; Bruker (UK) Ltd. Banner Lane Coventry UK.
  • Scherf T; Department of Chemical and Biological Physics Weizmann Institute of Science 7610001 Rehovot Israel.
  • Oxenfarth A; Institute for Organic Chemistry and Chemical Biology Center for Biomolecular Magnetic Resonance Johann Wolfgang Goethe-University 60438 Frankfurt/Main Germany.
  • Schnieders R; Institute for Organic Chemistry and Chemical Biology Center for Biomolecular Magnetic Resonance Johann Wolfgang Goethe-University 60438 Frankfurt/Main Germany.
  • Grün JT; Institute for Organic Chemistry and Chemical Biology Center for Biomolecular Magnetic Resonance Johann Wolfgang Goethe-University 60438 Frankfurt/Main Germany.
  • Wirmer-Bartoschek J; Institute for Organic Chemistry and Chemical Biology Center for Biomolecular Magnetic Resonance Johann Wolfgang Goethe-University 60438 Frankfurt/Main Germany.
  • Richter C; Institute for Organic Chemistry and Chemical Biology Center for Biomolecular Magnetic Resonance Johann Wolfgang Goethe-University 60438 Frankfurt/Main Germany.
  • Schwalbe H; Institute for Organic Chemistry and Chemical Biology Center for Biomolecular Magnetic Resonance Johann Wolfgang Goethe-University 60438 Frankfurt/Main Germany.
  • Frydman L; Department of Chemical and Biological Physics Weizmann Institute of Science 7610001 Rehovot Israel.
Angew Chem Weinheim Bergstr Ger ; 133(21): 11991-11998, 2021 May 17.
Article in English | MEDLINE | ID: covidwho-1381836
ABSTRACT
2D NOESY plays a central role in structural NMR spectroscopy. We have recently discussed methods that rely on solvent-driven exchanges to enhance NOE correlations between exchangeable and non-exchangeable protons in nucleic acids. Such methods, however, fail when trying to establish connectivities within pools of labile protons. This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations. The resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino-imino cross-peaks in RNAs, which is a first step in the NMR resolution of these structures. The origins of these enhancements are discussed, and their potential is demonstrated on RNA fragments derived from the genome of SARS-CoV-2, recorded with better sensitivity and an order of magnitude faster than conventional 2D counterparts.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Type of study: Randomized controlled trials Language: English Journal: Angew Chem Weinheim Bergstr Ger Journal subject: Biophysics / Chemistry Year: 2021 Document Type: Article

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Type of study: Randomized controlled trials Language: English Journal: Angew Chem Weinheim Bergstr Ger Journal subject: Biophysics / Chemistry Year: 2021 Document Type: Article